Yamamoto N, Naramoto S, Ohtani H
Department of Biomolecular Engineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
FEBS Lett. 1992 Dec 21;314(3):345-7. doi: 10.1016/0014-5793(92)81502-d.
Sophisticated measurements were made on the nanosecond time-resolved absorbance change of the purple membrane of Halobacterium halobium under cw background light irradiation (440-800 nm, 11-441 mW/cm2). A red-shifted transient species R660 (KN, Q) was found in alkaline conditions (pH > 9.3). Background light intensity effect shows that (i) R660 is photochemically formed from N560 intermediate which is accumulated under background light irradiation because of the elongated lifetime in alkaline suspension, and that (ii) the slow decaying M412 is not photochemically formed from N560 but from bR568.
在连续波背景光照射（440 - 800 nm，11 - 441 mW/cm²）下，对嗜盐嗜盐菌紫色膜的纳秒时间分辨吸光度变化进行了精密测量。在碱性条件（pH > 9.3）下发现了一个红移的瞬态物种R660（KN，Q）。背景光强度效应表明：（i）R660是由N560中间体光化学形成的，由于在碱性悬浮液中寿命延长，N560在背景光照射下积累；（ii）缓慢衰减的M412不是由N560光化学形成的，而是由bR568形成的。
