Lagarrigue S, Seillan-Heberden C, Martel P, Gaillard-Sanchez I
Laboratoire de Nutrition et de Sécurité Alimentaire, INRA, Jouy en Josas, France.
FEBS Lett. 1992 Dec 21;314(3):399-403. doi: 10.1016/0014-5793(92)81514-m.
Human c-fos cDNA was transfected into normal rat liver epithelial (REL) cells to identify cellular modifications associated with high expression of c-Fos protein. Responses to EGF and TGF beta were examined in the different cell lines, under anchorage-dependent and -independent conditions. Sensitivity to both factors was modified in transfected cells. While parental cells in monolayer did not respond to EGF, c-fos containing cells growth was stimulated by this factor. Overexpression of c-Fos protein led to an enhanced TGF beta-induced growth inhibition under anchorage dependent conditions, and TGF beta abolished spontaneous growth in soft agar of the cell lines containing c-fos oncogene. The mechanisms underlying the increased sensitivity to TGF beta in c-fos transfected cells are still to be determined.
将人c-fos cDNA转染到正常大鼠肝上皮（REL）细胞中，以鉴定与c-Fos蛋白高表达相关的细胞修饰。在不同细胞系中，在贴壁依赖性和非依赖性条件下检测对表皮生长因子（EGF）和转化生长因子β（TGFβ）的反应。转染细胞中对这两种因子的敏感性均发生了改变。单层培养的亲本细胞对EGF无反应，而含c-fos的细胞的生长受到该因子的刺激。在贴壁依赖性条件下，c-Fos蛋白的过表达导致TGFβ诱导的生长抑制增强，并且TGFβ消除了含c-fos癌基因的细胞系在软琼脂中的自发生长。c-fos转染细胞中对TGFβ敏感性增加的潜在机制仍有待确定。
