Yoshiura K, Ota S, Terano A, Takahashi M, Hata Y, Kawabe T, Mutoh H, Hiraishi H, Nakata R, Okano K
2nd Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Dig Dis Sci. 1994 Jul;39(7):1454-63. doi: 10.1007/BF02088048.
We recently developed a primary culture system for gastric epithelial cells from adult rabbits that allows the investigation of growth regulation at the cellular level. In this study, we demonstrated that epidermal growth factor (EGF), insulin, and dibutyryl adenosine 3',5'-cyclic monophosphate (dBcAMP) all stimulated cell proliferation. Insulin and dBcAMP potentiated the stimulation of cell proliferation by EGF, while transforming growth factor-beta 1 (TGF-beta 1) inhibited it. Expression of c-fos and c-myc was induced in response to the stimulation by these growth regulators, but the degree of expression did not necessarily correlate with the effects of these agents on cell proliferation. In conclusion, EGF, insulin, and dBcAMP were positive growth regulators, while TGF-beta 1 was a negative regulator in gastric epithelial cells. These growth modulators may exert their effects by distinct pathways from a standpoint of the expression of c-fos and c-myc.
我们最近开发了一种用于成年兔胃上皮细胞的原代培养系统，该系统能够在细胞水平上研究生长调节。在本研究中，我们证明表皮生长因子（EGF）、胰岛素和二丁酰腺苷3'，5'-环磷酸（dBcAMP）均能刺激细胞增殖。胰岛素和dBcAMP增强了EGF对细胞增殖的刺激作用，而转化生长因子-β1（TGF-β1）则抑制细胞增殖。这些生长调节剂刺激后诱导了c-fos和c-myc的表达，但表达程度不一定与这些因子对细胞增殖的影响相关。总之，EGF、胰岛素和dBcAMP是胃上皮细胞的正性生长调节剂，而TGF-β1是负性调节剂。从c-fos和c-myc表达的角度来看，这些生长调节剂可能通过不同的途径发挥作用。
