使用涡旋混合器采集睾丸精子。
Using a vortex mixer for testicular sperm collection.
作者信息
Tokuyama Osamu, Nakamura Yoshihiro, Ozaki Koji, Kawashima Kazumi, Fujino Yuji, Ishiko Osamu
机构信息
Department of Obstetrics and Gynecology, Osaka City University Medical School, Towako Reproduction Center Osaka, Osaka, Japan.
出版信息
J Reprod Med. 2003 Nov;48(11):865-8.
OBJECTIVE
To evaluate 2 methods of processing testicular tissue for the retrieval of viable sperm from men with nonobstructive azoospermia.
STUDY DESIGN
Fresh testicular tissue was obtained from nonobstructive azoospermia patients using a biopsy needle. The specimens were divided into 2 fractions. All specimens were minced and immersed in human tubal fluid (HTF). The first fraction was filtered through a nylon filter and incubated for 3 hours. The supernatant was centrifuged, resuspended in HTF and analyzed. The second fraction was immediately vortexed for 5 minutes and filtered through a nylon filter. The supernatant was centrifuged, resuspended in HTF and analyzed.
RESULTS
Spermatozoa were obtained in 13 of 24 cases (54.2%) using the vortex method and in 5 of 24 cases (20.8%) with the nylon filter method.
CONCLUSION
The vortex mixing method may be a better option than the conventional method for processing testicular tissue for sperm collection.
目的
评估两种处理睾丸组织的方法，以从非梗阻性无精子症男性中获取活精子。
研究设计
使用活检针从非梗阻性无精子症患者获取新鲜睾丸组织。标本分为两部分。所有标本均切碎并浸入人输卵管液（HTF）中。第一部分通过尼龙滤网过滤并孵育3小时。将上清液离心，重悬于HTF中并进行分析。第二部分立即涡旋5分钟并通过尼龙滤网过滤。将上清液离心，重悬于HTF中并进行分析。
结果
使用涡旋法在24例中有13例（54.2%）获得精子，使用尼龙滤网法在24例中有5例（20.8%）获得精子。
结论
对于处理睾丸组织以收集精子，涡旋混合法可能是比传统方法更好的选择。
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