Magdalena J, Forsman M, Lenzini M V, Brans A, Dusart J
Section of Genetics, Institut de Chimie, Liège, Belgium.
FEMS Microbiol Lett. 1992 Nov 15;78(1):101-5. doi: 10.1016/0378-1097(92)90295-y.
Two beta-lactamase genes called blaL and blaU have been cloned independently in Liège and in Umeå, from Streptomyces cacaoi. Genes blaL and blaU were found to differ largely in their nucleotide sequences, although the encoded proteins both belonged to the class A of beta-lactamases (active-site serine penicillinases). DNA-hybridization and polymerase chain reaction assays have now demonstrated that both blaL and blaU genes were present in the S. cacaoi strains used in Liège and in Umeå.
两个名为blaL和blaU的β-内酰胺酶基因已分别在列日和于默奥从可可链霉菌中独立克隆出来。尽管编码的蛋白质都属于A类β-内酰胺酶（活性位点丝氨酸青霉素酶），但发现blaL和blaU基因在核苷酸序列上有很大差异。DNA杂交和聚合酶链反应分析现已证明，列日和于默奥所使用的可可链霉菌菌株中均存在blaL和blaU基因。
