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可可链霉菌β-内酰胺酶BlaL的调控：blaB调控基因的产物是一种内膜结合蛋白。

Regulation of the beta-lactamase BlaL of Streptomyces cacaoi: the product of the blaB regulatory gene is an internal membrane-bound protein.

作者信息

Magdalena J, Joris B, Van Beeumen J, Brasseur R, Dusart J

机构信息

Centre d'Ingénierie des Protéines, Université de Liège, Belgium.

出版信息

Biochem J. 1995 Oct 1;311 ( Pt 1)(Pt 1):155-60. doi: 10.1042/bj3110155.

DOI:10.1042/bj3110155
PMID:7575447
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1136132/
Abstract

The beta-lactamase-encoding gene blaL, cloned from Streptomyces cacaoi in Streptomyces lividans, is inducible by beta-lactam compounds. This regulation has been shown to depend on the products of two open reading frames, ORF1 (blaA) and ORF2 (blaB) [Lenzini, Magdalena, Fraipont, Joris, Matagne and Dusart (1992) Mol. Gen. Genet. 235, 41-48]. BlaA belongs to the LysR family of transcription activators, whereas BlaB shares some features with the penicillin-recognizing proteins. BlaB has now been overexpressed in Escherichia coli, purified and used for antibody preparation. Immunoblotting of cell-fractionated materials from S. cacaoi showed that BlaB is attached to the internal face of the cytoplasmic membrane. It could not be released by high salt concentrations or EDTA, but only by protease treatment. Under the assay conditions, BlaB did not act as a penicillin-binding protein, a beta-lactamase, a D-amino-peptidase or a target in a phosphorylation step.

摘要

从可可链霉菌中克隆到变铅青链霉菌中的β-内酰胺酶编码基因blaL可被β-内酰胺类化合物诱导。已证明这种调控依赖于两个开放阅读框ORF1（blaA）和ORF2（blaB）的产物[伦齐尼、马格达莱纳、弗赖蓬、若里斯、马塔涅和迪萨尔（1992年）《分子与普通遗传学》235卷，41 - 48页]。BlaA属于转录激活因子的LysR家族，而BlaB与青霉素识别蛋白有一些共同特征。BlaB现已在大肠杆菌中过表达、纯化并用于抗体制备。对可可链霉菌细胞分级分离材料的免疫印迹分析表明，BlaB附着于细胞质膜的内表面。高盐浓度或EDTA不能将其释放，只有蛋白酶处理才能将其释放。在测定条件下，BlaB不作为青霉素结合蛋白、β-内酰胺酶、D - 氨基肽酶或磷酸化步骤中的靶点起作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40d7/1136132/cb0ab290e436/biochemj00054-0156-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40d7/1136132/ebafdd4ff42d/biochemj00054-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40d7/1136132/3db6b03a9060/biochemj00054-0156-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40d7/1136132/cb0ab290e436/biochemj00054-0156-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40d7/1136132/ebafdd4ff42d/biochemj00054-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40d7/1136132/3db6b03a9060/biochemj00054-0156-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40d7/1136132/cb0ab290e436/biochemj00054-0156-b.jpg

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