Poitevin-Later F, Vandenesch F, Dyke K, Fleurette J, Etienne J
Hôpital Louis Pradel, Laboratoire de Bactériologie, Lyon, France.
FEMS Microbiol Lett. 1992 Nov 15;78(1):59-63. doi: 10.1016/0378-1097(92)90288-y.
Plasmids that confer resistance to cadmium (MIC > or = 125 microM), were found in 18 out of 30 independent Staphylococcus lugdunensis strains from clinical specimens. Variants that were cured of their plasmid were cadmium sensitive. Restriction endonuclease sites of a 3.2-kb cadmium-resistance plasmid of S. lugdunensis, designated pLUG10, were similar to those of pOX6, a S. aureus cadmium-resistance plasmid containing the cadB gene. Southern-blot hybridisation was performed with a probe intragenic to cadB. Hybridisation was found with the cadB probe in the cadmium-resistant S. lugdunensis isolates to the 2.9-, 3.2- and 3.7-kb plasmids. These findings suggest that cadmium-resistance in some S. lugdunensis strains is due to a gene sharing homology with the cadB gene of S. aureus.
在从临床标本中分离出的30株独立的路邓葡萄球菌菌株中，有18株含有对镉具有抗性的质粒（最低抑菌浓度≥125微摩尔）。质粒消除后的变体对镉敏感。路邓葡萄球菌的一个3.2kb镉抗性质粒（命名为pLUG10）的限制性内切酶位点，与含有cadB基因的金黄色葡萄球菌镉抗性质粒pOX6的位点相似。用cadB基因内部的探针进行Southern杂交。在耐镉的路邓葡萄球菌分离株中，发现cadB探针与2.9kb、3.2kb和3.7kb的质粒发生杂交。这些发现表明，一些路邓葡萄球菌菌株的镉抗性是由于与金黄色葡萄球菌cadB基因具有同源性的基因所致。
