Udo E E, Grubb W B
School of Biomedical Sciences, Curtin University of Technology, Perth, Australia.
Curr Microbiol. 1995 Aug;31(2):71-6. doi: 10.1007/BF00294278.
Staphylococcus aureus isolate, WBG1022, was resistant to penicillin, kanamycin, neomycin, streptomycin, chloramphenicol, trimethoprim, cadmium, and ethidium bromide and harbored plasmids of 34.5, 24.5, 4.4, 3.2, and 2.6 kilobases. The plasmids were transferred in mixed-culture transfer and conjugation experiments. No resistance phenotype was associated with the 2.6-kb plasmid. The 3.2-kb and 4.4-kb plasmids encoded chloramphenicol and streptomycin resistance respectively. The 24.5-kb plasmid, pWBG626, encoded joint resistance to penicillin, kanamycin, neomycin, and ethidium bromide. Resistance to trimethoprim and cadmium were chromosomal. The 34.5-kb plasmid, pWBG661, had no resistance phenotype but was found to be conjugative. It also mobilized the 4.4-kb and 24.5-kb plasmids in WBG1022. Restriction endonuclease analysis of pWBG661 with EcoRI, ClaI, PvuII, and BglII restriction enzymes demonstrated that pWBG661 was identical to two previously isolated S. aureus conjugative plasmids, pWBG620 and pWBG637, that also lack resistance phenotypes.
金黄色葡萄球菌分离株WBG1022对青霉素、卡那霉素、新霉素、链霉素、氯霉素、甲氧苄啶、镉和溴化乙锭耐药，并且携带34.5、24.5、4.4、3.2和2.6千碱基的质粒。这些质粒在混合培养转移和接合实验中能够转移。2.6千碱基的质粒未表现出耐药表型。3.2千碱基和4.4千碱基的质粒分别编码氯霉素和链霉素耐药性。24.5千碱基的质粒pWBG626编码对青霉素、卡那霉素、新霉素和溴化乙锭的联合耐药性。对甲氧苄啶和镉的耐药性是由染色体介导的。34.5千碱基的质粒pWBG661没有耐药表型，但具有接合性。它还能在WBG1022中转移4.4千碱基和24.5千碱基的质粒。用EcoRI、ClaI、PvuII和BglII限制性内切酶对pWBG661进行限制性内切酶分析表明，pWBG661与之前分离的两个金黄色葡萄球菌接合性质粒pWBG620和pWBG637相同，这两个质粒也没有耐药表型。
