Xiong L, Li J, Kong F
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
Lett Appl Microbiol. 2004;38(1):32-7. doi: 10.1046/j.1472-765x.2003.01437.x.
To find new insecticidal antibiotics from marine micro-organisms.
Strains isolated from seawater and sea sediments from Beidiahe and Dagang of the east coast of China were screened for their insecticidal qualities. The screening was carried out using bioassay of brine shrimp and the insect pest Helicoverpa armigera. The fermentation, preliminary extraction and isolation of Streptomyces sp.173 were carried out.
In total 331 isolates were examined through bioassay of brine shrimp and 40 isolates (12.08%) showed potential insecticidal activities. Of the 40 isolates, one isolate, designated Streptomyces sp.173, was found to have strong insecticidal activity against both brine shrimp and H. armigera, similar to that of avermectin B1.
The isolated Streptomyces sp.173 has great insecticidal potency. This work indicated that marine micro-organisms could be an important source of insecticidal antibiotics and the improved anti-brine shrimp bioassay is suitable for primary screening.
从海洋微生物中寻找新的杀虫抗生素。
对从中国东海岸北戴河和大港的海水及海泥中分离出的菌株进行杀虫特性筛选。采用卤虫和棉铃虫生物测定法进行筛选。对链霉菌sp.173进行了发酵、初步提取和分离。
通过卤虫生物测定法共检测了331株分离物,40株(12.08%)显示出潜在的杀虫活性。在这40株分离物中,有一株命名为链霉菌sp.173的分离物,对卤虫和棉铃虫均具有较强的杀虫活性,与阿维菌素B1相似。
分离出的链霉菌sp.173具有很强的杀虫效力。这项工作表明海洋微生物可能是杀虫抗生素的重要来源,改进后的抗卤虫生物测定法适用于初步筛选。