痢疾志贺氏菌7型O抗原基因簇的结构及其抗原特异性基因的鉴定。
Structure of the Shigella dysenteriae 7 O antigen gene cluster and identification of its antigen specific genes.
作者信息
Feng Lu, Tao Jiang, Guo Hongjie, Xu Jianguo, Li Yayue, Rezwan Fedousi, Reeves Peter, Wang Lei
机构信息
College of Life Sciences, Nankai University, Tianjin, China.
出版信息
Microb Pathog. 2004 Feb;36(2):109-15. doi: 10.1016/j.micpath.2003.10.003.
Shigella strains are human pathogens. The O antigen gene cluster of Shigella dysenteriae O7 was sequenced and analyzed. It contains genes for synthesis of nucleotide sugars including UDP-2-acetamido-2-deoxy-D-galacturonamide, UDP-2-acetamido-2-deoxy-D-galacturonic acid and dTDP-4-amino-4,6-dideoxy-D-glucose. Also found in the gene cluster are genes encoding O unit flippase, O antigen polymerase and sugar transferases. The Escherichia coli O121 O antigen, which is present in an important Shiga toxin-producing strain, has the same structure as that of S. dysenteriae O7, and we found that the gene clusters also had the same genes and organization. Four genes specific to S. dysenteriae O7 and E. coli O121 were identified by PCR screening against representatives of 186 E. coli (including Shigella) O serotypes. E. coli O121 and S. dysenteriae O7 isolates can be distinguished by PCR of the H antigen fliC gene.
志贺氏菌菌株是人类病原体。对痢疾志贺氏菌O7的O抗原基因簇进行了测序和分析。它包含用于合成核苷酸糖的基因，包括UDP-2-乙酰氨基-2-脱氧-D-半乳糖醛酰胺、UDP-2-乙酰氨基-2-脱氧-D-半乳糖醛酸和dTDP-4-氨基-4,6-二脱氧-D-葡萄糖。在该基因簇中还发现了编码O单位翻转酶、O抗原聚合酶和糖基转移酶的基因。存在于一种重要的产志贺毒素菌株中的大肠杆菌O121 O抗原与痢疾志贺氏菌O7的结构相同，并且我们发现基因簇也具有相同的基因和组织。通过针对186种大肠杆菌（包括志贺氏菌）O血清型的代表进行PCR筛选，鉴定出了四个痢疾志贺氏菌O7和大肠杆菌O121特有的基因。大肠杆菌O121和痢疾志贺氏菌O7分离株可以通过H抗原fliC基因的PCR进行区分。
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