脊柱裂胎儿和对照胎儿羊水的高分辨率¹H核磁共振波谱分析。
High-resolution 1H NMR spectroscopy of amniotic fluids from spina bifida fetuses and controls.
作者信息
Groenen Pascal M W, Engelke Udo F, Wevers Ron A, Hendriks Jan C M, Eskes Tom K A B, Merkus Hans M W M, Steegers-Theunissen Régine P M
机构信息
Department of Epidemiology and Biostatistics, University Medical Center Nijmegen, Nijmegen, The Netherlands.
出版信息
Eur J Obstet Gynecol Reprod Biol. 2004 Jan 15;112(1):16-23. doi: 10.1016/s0301-2115(03)00279-3.
OBJECTIVE
To quantify proton containing metabolites by in vitro 1H NMR spectroscopy of amniotic fluids from fetuses with spina bifida and controls.
STUDY DESIGN
Fourteen amniotic fluids from spina bifida fetuses and 18 controls were obtained. Concentrations of carbohydrates, organic acids and amino acids were determined. Multiple linear regression analysis was used to evaluate the data.
RESULTS
At 15 and 39 weeks amenorrhea, the estimated median amniotic fluid concentrations of succinic acid and glutamine were significantly higher in the spina bifida group compared to controls, 37 and 64%, respectively. Whereas creatine and creatinine were significantly lower, 27 and 36%, respectively. Amenorrhea influenced the concentrations of most compounds with the exception of lactic acid.
CONCLUSION
1H NMR spectroscopy shows significantly higher succinic acid and glutamine concentrations in amniotic fluids derived from spina bifida fetuses compared with controls. A derangement in amino acid metabolism is suggested.
目的
通过对脊柱裂胎儿和对照组胎儿羊水进行体外1H核磁共振波谱分析，对含质子代谢物进行定量分析。
研究设计
获取了14份脊柱裂胎儿的羊水样本和18份对照样本。测定了碳水化合物、有机酸和氨基酸的浓度。采用多元线性回归分析对数据进行评估。
结果
在停经15周和39周时，脊柱裂组羊水中琥珀酸和谷氨酰胺的估计中位浓度显著高于对照组，分别高出37%和64%。而肌酸和肌酐则显著降低，分别降低27%和36%。停经影响了大多数化合物的浓度，乳酸除外。
结论
1H核磁共振波谱分析显示，与对照组相比，脊柱裂胎儿羊水中琥珀酸和谷氨酰胺的浓度显著更高。提示氨基酸代谢紊乱。
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