Lichtor T, Getz G S
Proc Natl Acad Sci U S A. 1978 Jan;75(1):324-8. doi: 10.1073/pnas.75.1.324.
Mouse fibroblasts resistant to the drug rutamycin were isolated by selectively introducing BrdUrd into the mitochondrial genome of a line of mouse fibroblasts (clone 1 D) lacking a cytoplasmic thymidine kinase enzyme. The ATPase (ATP phosphohydrolase; EC 3.6.1.3) activity of mitochondria isolated from these cells was resistant to rutamycin. The rutamycin-resistant mutants were enucleated with cytochalasin B and fused with mouse A 9 cells resistant to 8-azaguanine and sensitive to rutamycin. Cytoplasmic hybrids, or cybrids, were selected as cells resistant to rutamycin and 8-azaguanine, and appeared at a high frequency. Other fusions between rutamycin-resistant nucleated cells and A 9 produced colonies at a much lower frequency. Finally, fusions between enucleated clone 1 D cells and A 9 cells produced no rutamycin-resistant colonies. These results indicate that rutamycin resistance is a cytoplasmically inherited characteristic in this cell line.
通过选择性地将溴脱氧尿苷引入缺乏细胞质胸苷激酶的小鼠成纤维细胞系（克隆1D）的线粒体基因组中，分离出对药物鲁塔霉素具有抗性的小鼠成纤维细胞。从这些细胞中分离出的线粒体的ATP酶（ATP磷酸水解酶；EC 3.6.1.3）活性对鲁塔霉素具有抗性。用细胞松弛素B去除鲁塔霉素抗性突变体的细胞核，并与对8-氮杂鸟嘌呤具有抗性且对鲁塔霉素敏感的小鼠A9细胞融合。选择细胞质杂种细胞（即胞质杂种细胞）作为对鲁塔霉素和8-氮杂鸟嘌呤具有抗性的细胞，其出现频率很高。鲁塔霉素抗性有核细胞与A9细胞之间的其他融合产生菌落的频率要低得多。最后，去核的克隆1D细胞与A9细胞之间融合未产生鲁塔霉素抗性菌落。这些结果表明，在该细胞系中，鲁塔霉素抗性是一种细胞质遗传特征。
