与小鼠畸胎癌细胞发生反应的单克隆抗体。
Monoclonal antibodies reacting with murine teratocarcinoma cells.
作者信息
Goodfellow P N, Levinson J R, Williams V E, McDevitt H O
出版信息
Proc Natl Acad Sci U S A. 1979 Jan;76(1):377-80. doi: 10.1073/pnas.76.1.377.
Abstract
Monoclonal antibodies were produced in vitro by fusing mouse myeloma cells with spleen cells from a rat immunized with the C3H mouse teratocarcinoma C86-S1. After the fusion two clones were chosen for further analysis. The first clone, 3C4-10, produced an antibody recognizing an antigen with a distribution restricted to teratocarcinoma cell lines, an endoderm cell line, and a neuroblastoma. The second clone, 4A1-9, produced an antibody that reacted with all cultured murine cells tested and adult brain. Neither antibody reacted with preimplantation embryos. The 3C4-10 antibody recognized an antigen associated with proteins. The apparent molecular weight of the 3C4-10 antigen was greater than 100,000.
摘要
通过将小鼠骨髓瘤细胞与用C3H小鼠畸胎癌C86 - S1免疫的大鼠脾细胞融合，在体外产生单克隆抗体。融合后选择两个克隆进行进一步分析。第一个克隆3C4 - 10产生一种抗体，该抗体识别一种抗原，其分布仅限于畸胎癌细胞系、一种内胚层细胞系和一种神经母细胞瘤。第二个克隆4A1 - 9产生一种抗体，该抗体与所有测试的培养鼠细胞和成年脑反应。两种抗体均不与植入前胚胎反应。3C4 - 10抗体识别一种与蛋白质相关的抗原。3C4 - 10抗原的表观分子量大于100,000。
相似文献
1
Monoclonal antibodies reacting with murine teratocarcinoma cells.与小鼠畸胎癌细胞发生反应的单克隆抗体。
Proc Natl Acad Sci U S A. 1979 Jan;76(1):377-80. doi: 10.1073/pnas.76.1.377.
2
Monoclonal antibody detecting a stage-specific embryonic antigen (SSEA-1) on preimplantation mouse embryos and teratocarcinoma cells.一种单克隆抗体，可检测植入前小鼠胚胎和畸胎瘤细胞上的阶段特异性胚胎抗原（SSEA-1）。
Curr Top Microbiol Immunol. 1978;81:51-3. doi: 10.1007/978-3-642-67448-8_8.
3
Independent regulation of H-2K and H-2D gene expression in murine teratocarcinoma somatic cell hybrids.小鼠畸胎瘤体细胞杂种中H-2K和H-2D基因表达的独立调控
J Exp Med. 1980 Jun 1;151(6):1349-59. doi: 10.1084/jem.151.6.1349.
4
Serological analysis of early mouse embryo with rat monoclonal antibodies produced against mouse teratocarcinoma cells.用针对小鼠畸胎瘤细胞产生的大鼠单克隆抗体对早期小鼠胚胎进行血清学分析。
Differentiation. 1985;28(3):260-7. doi: 10.1111/j.1432-0436.1985.tb00834.x.
5
A monoclonal antibody to the murine teratoma, F9: an immunocytochemical demonstration of tissue specificity.一种针对小鼠畸胎瘤F9的单克隆抗体：组织特异性的免疫细胞化学证明
Diagn Histopathol. 1981 Apr-Jun;4(2):129-35.
6
Monoclonal antibodies to teratomas and breast.针对畸胎瘤和乳腺癌的单克隆抗体。
Transplant Proc. 1980 Sep;12(3):398-402.
7
Monoclonal antibody to murine embryos defines a stage-specific embryonic antigen expressed on mouse embryos and human teratocarcinoma cells.针对小鼠胚胎的单克隆抗体可识别一种在小鼠胚胎和人畸胎癌细胞上表达的阶段特异性胚胎抗原。
Cell. 1982 Oct;30(3):697-705. doi: 10.1016/0092-8674(82)90274-4.
8
Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).定义一种阶段特异性小鼠胚胎抗原（SSEA-1）的单克隆抗体。
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5565-9. doi: 10.1073/pnas.75.11.5565.
9
Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells.三种单克隆抗体，它们定义了与人类胚胎癌细胞表面不同的高分子量多肽相关的独特分化抗原。
Hybridoma. 1984 Winter;3(4):347-61. doi: 10.1089/hyb.1984.3.347.
10
Monoclonal antibodies specific for carcinoembryonic antigen and produced by two hybrid cell lines.由两个杂交细胞系产生的、对癌胚抗原有特异性的单克隆抗体。
Proc Natl Acad Sci U S A. 1980 Jan;77(1):563-6. doi: 10.1073/pnas.77.1.563.
引用本文的文献
1
Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue.在神经元和神经分泌组织中广泛分布的一种突触小泡特异性膜蛋白的鉴定。
J Cell Biol. 1981 Oct;91(1):257-69. doi: 10.1083/jcb.91.1.257.
2
Immunologic recognition of cell surface antigens in normal mouse neural tissues and in neuroepithelial cells of the OTT-6050 mouse teratoma. A radiometric, gel electrophoretic and morphologic (immunofluorescence and immunoperoxidase) study.正常小鼠神经组织及OTT-6050小鼠畸胎瘤神经上皮细胞中细胞表面抗原的免疫识别。一项放射性测定、凝胶电泳及形态学（免疫荧光和免疫过氧化物酶）研究。
Acta Neuropathol. 1982;56(3):214-24. doi: 10.1007/BF00690638.
3
Characterization of a new human cell line derived from a xenografted embryonal carcinoma.源自异种移植胚胎癌的一种新人类细胞系的特性分析。
In Vitro. 1982 Sep;18(9):739-49. doi: 10.1007/BF02796497.
4
Transformation-related antigens identified by monoclonal antibodies.通过单克隆抗体鉴定的转化相关抗原。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3234-8. doi: 10.1073/pnas.77.6.3234.
5
The glycosidic antigen recognised by a novel monoclonal antibody, 75.12, is developmentally regulated on mouse embryonal carcinoma cells.一种新型单克隆抗体75.12所识别的糖苷抗原在小鼠胚胎癌细胞上受到发育调控。
EMBO J. 1983;2(12):2217-22. doi: 10.1002/j.1460-2075.1983.tb01726.x.
6
Isolation and quantitation of the platelet membrane glycoprotein deficient in thrombasthenia using a monoclonal hybridoma antibody.使用单克隆杂交瘤抗体分离和定量血小板无力症中缺乏的血小板膜糖蛋白。
J Clin Invest. 1980 Dec;66(6):1311-8. doi: 10.1172/JCI109983.
7
Monoclonal antibodies against beta nerve growth factor and their effects on receptor binding and biological activity.抗β神经生长因子单克隆抗体及其对受体结合和生物活性的影响。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4611-5. doi: 10.1073/pnas.78.7.4611.
8
Whither monoclonal antibodies?单克隆抗体何去何从？
Immunology. 1980 Jul;40(3):287-93.
9
Hybridomas: a new dimension in biological analyses.杂交瘤：生物学分析的新维度。
In Vitro. 1981 Dec;17(12):1036-50. doi: 10.1007/BF02618601.
10
Immunohistochemical localization of murine stage-specific embryonic antigens in human testicular germ cell tumors.鼠类阶段特异性胚胎抗在人睾丸生殖细胞肿瘤中的免疫组织化学定位
Am J Pathol. 1982 Aug;108(2):225-30.
本文引用的文献
1
Detection of H-2 antigens on mouse spermatozoa by the cytotoxicity test.通过细胞毒性试验检测小鼠精子上的H-2抗原。
Nature. 1970 Nov 7;228(5271):570-2. doi: 10.1038/228570a0.
2
Establishment of functional clonal lines of neurons from mouse neuroblastoma.从小鼠神经母细胞瘤建立功能性神经元克隆系。
Proc Natl Acad Sci U S A. 1969 Sep;64(1):311-5. doi: 10.1073/pnas.64.1.311.
3
Neoplastic differentiation: characteristics of cell lines derived from a murine teratocarcinoma.肿瘤分化：源自小鼠畸胎癌的细胞系特征
J Cell Physiol. 1974 Aug;84(1):13-27. doi: 10.1002/jcp.1040840103.
4
[Teratocarcinoma of the mouse: isolation, culture and properties of pluripotential cells].[小鼠畸胎癌：多能细胞的分离、培养及特性]
Ann Microbiol (Paris). 1973 Oct;124(3):269-82.
5
The binding of anti-immunoglobulin antibodies to rat thymocytes and thoracic duct lymphocytes.抗免疫球蛋白抗体与大鼠胸腺细胞和胸导管淋巴细胞的结合。
Eur J Immunol. 1974 Feb;4(2):91-7. doi: 10.1002/eji.1830040207.
6
Complementation patterns of Thy-1 variants and evidence that antigen loss variants "pre-exist" in the parental population.Thy-1变体的互补模式以及抗原缺失变体在亲代群体中“预先存在”的证据。
J Natl Cancer Inst. 1974 Feb;52(2):429-36. doi: 10.1093/jnci/52.2.429.
7
Alkaline phosphatase activity in mouse teratoma.小鼠畸胎瘤中的碱性磷酸酶活性。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3899-903. doi: 10.1073/pnas.70.12.3899.
8
Cell surface antigens of a mouse testicular teratoma. Identification of an antigen physically associated with H-2 antigens on tumor cells.小鼠睾丸畸胎瘤的细胞表面抗原。肿瘤细胞上与H-2抗原物理相关的一种抗原的鉴定。
J Exp Med. 1974 Jul 1;140(1):61-78. doi: 10.1084/jem.140.1.61.
9
Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture.培养的小鼠卵裂期胚胎和原始性畸胎瘤细胞共有的表面抗原。
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2988-92. doi: 10.1073/pnas.70.10.2988.
10
Immunologic cross-reactivity between antigen of unfertilized mouse eggs and mouse cells transformed by simian virus 40.未受精小鼠卵抗原与经猿猴病毒40转化的小鼠细胞之间的免疫交叉反应性。
J Natl Cancer Inst. 1971 Jun;46(6):1317-23.
Zotero 插件