Foury F, Tzagoloff A
Eur J Biochem. 1976 Sep;68(1):113-9. doi: 10.1111/j.1432-1033.1976.tb10769.x.
Four cytoplasmic mutants of Saccharomyces cerevisiae showing loss of mitochondrial rutamycin-sensitive ATPase activity but having significant cytochrome oxidase and NADH-cytochrome c reductase have been isolated. Genetic studies indicate the mutations to be closely linked to each other and have been assigned to a new locus, PHO1. The mutations show a low frequency of recombination with the OL12 locus, suggesting a linkage to this marker. They are not, however, linked to the OLI1 locus. Linkage of the ATPase mutations to the OLI2 locus is also indicated by restoration of wild-type diploids by sigma- clones that retain the segment of mitochondrial DNA carrying OLI2. Based on the recombinants issued from crosses of the mutants with a triple drug-resistant strain and an analysis of the resistance markers present in sigma- clones that are effective in restoring a wild-type phenotype, the PHO1 locus has been placed in the segment of DNA located between PAR1 and OLI2.
已分离出酿酒酵母的四个细胞质突变体，它们表现出线粒体鲁塔霉素敏感型ATP酶活性丧失，但细胞色素氧化酶和NADH-细胞色素c还原酶活性显著。遗传学研究表明，这些突变彼此紧密连锁，并已被定位到一个新的基因座PHO1。这些突变与OL12基因座的重组频率较低，表明与该标记存在连锁关系。然而，它们与OLI1基因座不连锁。保留携带OLI2的线粒体DNA片段的σ克隆恢复野生型二倍体，这也表明ATP酶突变与OLI2基因座存在连锁关系。基于突变体与三抗菌株杂交产生的重组体，以及对能有效恢复野生型表型的σ克隆中存在的抗性标记的分析，PHO1基因座已被定位到位于PAR1和OLI2之间的DNA片段中。
