Del Campo J A, Rodríguez H, Moreno J, Vargas M A, Rivas J, Guerrero M G
Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Avda. Américo Vespucio s/n, 41092 Sevilla, Spain.
Appl Microbiol Biotechnol. 2004 Jun;64(6):848-54. doi: 10.1007/s00253-003-1510-5. Epub 2003 Dec 20.
When grown photoautotrophically, Chlorella zofingiensis strain CCAP 211/14 accumulates a significant amount of valuable carotenoids, namely astaxanthin and lutein, of increasing demand for use as feed additives in fish and poultry farming, as colorants in food, and in health care products. Under standard batch-culture conditions, this microalgal strain exhibits high values of both growth rate (about 0.04 h(-1)) and standing cell population (over 10(11) cells l(-1), or 7 g dry weight l(-1)). Lutein, in a free (unesterified) form, was the prevalent carotenoid during early stages of cultivation (over 0.3 pg cell(-1), equal to 4 mg g(-1) dry weight, or 20 mg l(-1) culture), whereas esterified astaxanthin accumulated progressively, to reach a maximum (over 0.1 pg cell(-1), equal to 1.5 mg g(-1) dry weight, or 15 mg l(-1) culture) in the late stationary phase. A differential response of lutein and astaxanthin accumulation was also recorded with regard to the action of some environmental and nutritional factors. C. zofingiensis CCAP 211/14 represents a unique model system for analyzing the differential regulation of the levels of primary (lutein) and secondary (astaxanthin) carotenoids. Relevant also from the biotechnological viewpoint, this photosynthetic organism, with outstanding attributes for fast photosynthetic growth and carotenoid accumulation, might prove most valuable for its application to the mass production of either or both lutein and astaxanthin.
当在光自养条件下培养时，佐夫小球藻CCAP 211/14菌株会积累大量有价值的类胡萝卜素，即虾青素和叶黄素，它们在鱼类和家禽养殖中用作饲料添加剂、食品中的色素以及保健品中的需求日益增加。在标准分批培养条件下，这种微藻菌株的生长速率（约0.04 h⁻¹）和细胞静置密度（超过10¹¹个细胞/升，或7克干重/升）都很高。在培养早期，游离（未酯化）形式的叶黄素是主要的类胡萝卜素（超过0.3 pg/细胞，相当于4毫克/克干重，或20毫克/升培养物），而酯化虾青素则逐渐积累，在稳定期末期达到最大值（超过0.1 pg/细胞，相当于1.5毫克/克干重，或15毫克/升培养物）。关于一些环境和营养因素的作用，还记录到了叶黄素和虾青素积累的差异反应。佐夫小球藻CCAP 211/14是分析初级（叶黄素）和次级（虾青素）类胡萝卜素水平差异调节的独特模型系统。从生物技术的角度来看也很重要，这种光合生物具有快速光合生长和类胡萝卜素积累的突出特性，可能在叶黄素和虾青素的大规模生产中具有极高的应用价值。
