Bleisch Richard, Ihadjadene Yob, Torrisi Agnese, Walther Thomas, Mühlstädt Gunnar, Steingröwer Juliane, Streif Stefan, Krujatz Felix
Institute of Natural Materials Technology, Technische Universität Dresden, 01069 Dresden, Germany.
Chair of Automatic Control & System Dynamics, Technische Universität Chemnitz, 09126 Chemnitz, Germany.
Life (Basel). 2025 Apr 14;15(4):648. doi: 10.3390/life15040648.
is a green alga that serves as a valuable source of lipids, proteins, and carotenoids. Compared to well-studied microalgal carotenoid producers, offers several advantages, including high biomass, lipid and carotenoid productivity as well as less susceptibility to contaminations. can achieve growth rates up to four times higher than those of under optimal phototrophic conditions. Although several studies have examined its cultivation and carotenogenesis under different tropic growth modes at laboratory scale, few have focused on pilot-scale systems. The goal of this study is to investigate the microalga's physiological adaptation in a 200 L tubular photobioreactor during a three-phase semi-continuous cultivation strategy, particularly focusing on the changes in macromolecular and pigment composition. After an initial biomass accumulation phase, a two-phased stress phase was applied combining nutrient depletion (phase 1) and osmotic salt stress conditions (phase 2). Following this procedure, the cellular protein content dropped to 44.7% of its initial level, while the lipid content rose by up to 320%. Additionally, the astaxanthin concentration increased from 1.1 mg/g to 4.9 mg/g during the last osmotic stress phases, aligning with results from published laboratory-scale studies.
是一种绿藻，是脂质、蛋白质和类胡萝卜素的宝贵来源。与经过充分研究的微藻类胡萝卜素生产者相比，具有几个优势，包括高生物量、脂质和类胡萝卜素生产率以及对污染的敏感性较低。在最佳光养条件下，的生长速度可达的四倍。尽管有几项研究在实验室规模下研究了其在不同营养生长模式下的培养和类胡萝卜素生成，但很少有研究关注中试规模系统。本研究的目的是调查微藻在200升管式光生物反应器中采用三相半连续培养策略时的生理适应性，特别关注大分子和色素组成的变化。在初始生物量积累阶段之后，应用了一个两阶段胁迫阶段，结合营养耗尽（阶段1）和渗透盐胁迫条件（阶段2）。按照此程序，细胞蛋白质含量降至初始水平的44.7%，而脂质含量则上升高达320%。此外，在最后一个渗透胁迫阶段，虾青素浓度从1.1毫克/克增加到4.9毫克/克，与已发表的实验室规模研究结果一致。
