Fidalgo M L, Arias M S, Soliveri J, Arias M E
Departamento de Microbiología y Parasitología, Universidad de Alcalá de Henares, Madrid, Spain.
J Antibiot (Tokyo). 1992 Nov;45(11):1759-62. doi: 10.7164/antibiotics.45.1759.
Two new pyrazolo-isoquinolinone antibiotics, APHE-1 and APHE-2, have been isolated from the culture filtrate and mycelia of Streptoverticillium griseocarneum NCIMB 40447. Molecular formulae were established as C13H12N2O for APHE-1 and C14H14N2O for APHE-2, by elemental analysis, NMR and mass spectra. 2D NMR techniques (1H-1H COSY-45 and 1H-13C correlated spectroscopy) have been applied to establish their structures.