一种从单个犬冠状动脉中分离内皮细胞和平滑肌细胞的逐步方法。
A stepwise method for the isolation of endothelial cells and smooth muscle cells from individual canine coronary arteries.
作者信息
Dame Michael K, Yu Xinwen, Garrido Rosario, Bobrowski Walter, McDuffie J Eric, Murphy Hedwig S, Albassam Mudher, Varani James
机构信息
Department of Pathology, University of Michigan Medical School, 4224 Medical Science I Building, 1301 Catherine Street, Ann Arbor, Michigan 48109-0602, USA.
出版信息
In Vitro Cell Dev Biol Anim. 2003 Nov-Dec;39(10):402-6. doi: 10.1290/1543-706X(2003)039<0402:ASMFTI>2.0.CO;2.
Methods for the stepwise isolation of endothelial cells and smooth muscle cells from individual canine coronary arteries are described. Both cell types can be isolated in pure culture with high yields. Dogs are a common species used in the study of atherosclerosis and coronary artery disease. Capacity to isolate endothelial cells and smooth muscle cells from individual canine coronary arteries should prove useful in the study of coronary artery disease.
本文描述了从单个犬冠状动脉逐步分离内皮细胞和平滑肌细胞的方法。两种细胞类型都可以在纯培养物中高产率地分离出来。犬是动脉粥样硬化和冠状动脉疾病研究中常用的物种。从单个犬冠状动脉中分离内皮细胞和平滑肌细胞的能力在冠状动脉疾病研究中应会被证明是有用的。
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