Yasuoka Takashi, Sasaki Masaya, Fukunaga Tetsuya, Tsujikawa Tomoyuki, Fujiyama Yoshihide, Kushima Ryouji, Goodlad Robert A
Department of Gastroenterology, Shiga University of Medical Science, Otsu, Japan.
Int J Exp Pathol. 2003 Oct;84(5):231-7. doi: 10.1111/j.1365-2613.2003.00360.x.
Growth factors, such as epidermal growth factor and keratinocyte growth factor, have considerable therapeutic potential for repairing mucosal injury in the intestine when given systemically. Recently, several lectins have been shown to have trophic effects on the intestine when given orally. We examined the effects of phytohaemagglutinin (PHA) and concanavalin A (Con-A) on indomethacin-induced intestinal injury in rat. Five-week-old rats were randomized to four groups (n=5), and intestinal injury was induced by indomethacin injection in three of these groups. Elemental diet (ED) feeding was then commenced. The groups were thus ED feeding/indomethacin untreated (control group), ED feeding/indomethacin treated (ED group), 0.1% PHA-supplemented ED feeding/indomethacin treated (PHA group) and 0.1% Con-A-supplemented ED feeding/indomethacin treated (Con-A group). After 7 days of feeding, macroscopic inflammatory scores, mucosal permeability, myeloperoxidase (MPO) activities and cell proliferation were determined. Macroscopic inflammatory scores, mucosal permeability and MPO activities were significantly lower in both lectin groups than that in control group. Twenty-four hour excretion rate of phenolsulphonphthalein was significantly lower in both lectin groups than that in ED group. Cell proliferation of the small intestine was significantly increased by both lectins. Lectin supplementation can induce ulcer healing following indomethacin-induced damage.
生长因子，如表皮生长因子和角质形成细胞生长因子，全身给药时对修复肠道黏膜损伤具有相当大的治疗潜力。最近，有研究表明几种凝集素口服给药时对肠道具有营养作用。我们研究了植物血凝素（PHA）和伴刀豆球蛋白A（Con-A）对吲哚美辛诱导的大鼠肠道损伤的影响。将5周龄大鼠随机分为四组（每组n=5），其中三组通过注射吲哚美辛诱导肠道损伤。然后开始给予要素饮食（ED）。分组如下：ED喂养/未用吲哚美辛处理（对照组）、ED喂养/用吲哚美辛处理（ED组）、补充0.1%PHA的ED喂养/用吲哚美辛处理（PHA组）和补充0.1%Con-A的ED喂养/用吲哚美辛处理（Con-A组）。喂养7天后，测定宏观炎症评分、黏膜通透性、髓过氧化物酶（MPO）活性和细胞增殖情况。两个凝集素组的宏观炎症评分、黏膜通透性和MPO活性均显著低于对照组。两个凝集素组的酚红24小时排泄率均显著低于ED组。两种凝集素均显著增加了小肠的细胞增殖。补充凝集素可诱导吲哚美辛诱导损伤后的溃疡愈合。
