Gusman Heloisa, Leone Cataldo, Helmerhorst Eva J, Nunn Martha, Flora Bianca, Troxler Robert F, Oppenheim Frank G
Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 100 East Newton Street, Boston, MA 02118, USA.
Arch Oral Biol. 2004 Jan;49(1):11-22. doi: 10.1016/s0003-9969(03)00182-1.
Histatins constitute a distinct family of human salivary antimicrobial peptides, of which histatins 1, 3 and 5 are the most abundant. To evaluate salivary gland-specific differences in histatin secretion, we used the recently developed histatin-zinc precipitation method to quantify histatins and to assess daily variations in secretions. Stimulated pure secretions from parotid glands (HPS) and submandibular/sublingual glands (SMSL) were collected from 10 different subjects at four different times of the day (9:35 a.m.; 12:40 p.m.; 2:50 p.m. and 5:00 p.m.). Zinc precipitation and subsequent reversed phase HPLC analysis were performed to determine concentrations of histatins 1, 3 and 5 with reference to purified histatin standards. Both HPS and SMSL secretions displayed daily variations in histatin concentrations. HPS values showed a maximum at mid-day and SMSL samples showed a maximum in the morning. Mean daily histatin concentrations were almost three fold higher in SMSL than in HPS. Mean histatin 1, 3 and 5 concentrations in HPS from 10 subjects ranged from 0.7 to 2.8, 0.6 to 4.3 and 1.0 to 4.3mg%, respectively. The corresponding means in SMSL were 2.8-12.2, 1.5-7.5 and 2.6-9.0mg%, respectively. Remarkably, although histatins constitute only 3-10% of total protein in these secretions, an almost perfect correlation between total protein and total histatin concentrations was observed for both glands. Despite a broad range in histatin concentrations between individuals, this study demonstrated a hitherto unidentified daily variation in histatin concentrations in HPS and SMSL secretions and a differential expression pattern which might have functional implications.
组蛋白是人类唾液抗菌肽中的一个独特家族，其中组蛋白1、3和5最为丰富。为了评估唾液腺在组蛋白分泌方面的特异性差异，我们使用了最近开发的组蛋白-锌沉淀法来定量组蛋白，并评估分泌物的每日变化。在一天中的四个不同时间（上午9:35；下午12:40；下午2:50和下午5:00），从10名不同的受试者中收集腮腺（HPS）和颌下腺/舌下腺（SMSL）的刺激纯分泌物。进行锌沉淀和随后的反相高效液相色谱分析，以参照纯化的组蛋白标准品测定组蛋白1、3和5的浓度。HPS和SMSL分泌物中的组蛋白浓度均呈现每日变化。HPS的值在中午达到最高，而SMSL样本在早晨达到最高。SMSL中组蛋白的平均每日浓度几乎是HPS中的三倍。10名受试者的HPS中组蛋白1、3和5的平均浓度分别为0.7至2.8、0.6至4.3和1.0至4.3mg%。SMSL中的相应平均值分别为2.8 - 12.2、1.5 - 7.5和2.6 - 9.0mg%。值得注意的是，尽管组蛋白在这些分泌物中仅占总蛋白的3 - 10%，但在两个腺体中均观察到总蛋白与总组蛋白浓度之间几乎完美的相关性。尽管个体之间组蛋白浓度范围很广，但本研究证明了HPS和SMSL分泌物中组蛋白浓度迄今未被识别的每日变化以及可能具有功能意义的差异表达模式。
