Johnson D A, Yeh C K, Dodds M W
Department of Community Dentistry (7917), Dental School, University of Texas Health Science Center, San Antonio, TX 78229-3900, USA.
Arch Oral Biol. 2000 Sep;45(9):731-40. doi: 10.1016/s0003-9969(00)00047-9.
Histatins are small proteins of human glandular saliva that have antifungal properties. Recent studies show that oral candidal infections increase with age, suggesting an age-associated compromise in oral host defence. Here, the effect of age and of physiological gland stimulation on the concentration and secretion of salivary histatins was investigated. Parotid and submandibular/sublingual salivas were collected from six young adults under unstimulated, mechanical (chewing) and gustatory (0.025 M and 0.1 M citric acid) stimulation, and the concentration and secretion of histatins was measured by cationic polyacrylamide gel electrophoresis with subsequent densitometric scanning of the stained gels. With gland stimulation, parotid saliva showed no significant increase in histatin concentration (microg/ml); however, histatin secretion (microg/min) increased up to 26-fold (p<0.005; ANOVA). Stimulation of submandibular/sublingual saliva resulted in significant increases in both histatin concentration (p<0.005) and secretion (p<0.0005). Ageing effects on salivary histatins were determined in citric acid (0.1 M)-stimulated parotid and submandibular/sublingual saliva samples collected from 80 individuals (divided into four age groups having approximately equal numbers of males and females: 35-44 years; 45-54 years; 55-64 years and 65-76 years). None of the patients was taking medications or wore dentures. ANOVA showed no sex differences in histatins. Regression analysis showed significant age-associated decreases for parotid saliva histatin concentration (p<0.002) and secretion (p<0. 002) as well as for submandibular/sublingual saliva histatin concentration (p<0.0001) and secretion (p<0.0001). Both saliva types showed significant (p<0.0001) decreases in the histatin concentration per mg of total protein, suggesting a preferential decrease in salivary histatins compared to total salivary protein. These results suggest that the salivary histatin component of the oral host defence system is compromised with increasing age.
组蛋白是人类腺性唾液中的小蛋白质，具有抗真菌特性。最近的研究表明，口腔念珠菌感染随年龄增长而增加，这表明口腔宿主防御存在与年龄相关的损害。在此，研究了年龄和生理性腺体刺激对唾液组蛋白浓度和分泌的影响。从6名年轻成年人中收集腮腺和下颌下/舌下唾液，分别处于非刺激、机械性（咀嚼）和味觉性（0.025M和0.1M柠檬酸）刺激状态，通过阳离子聚丙烯酰胺凝胶电泳测定组蛋白的浓度和分泌量，随后对染色凝胶进行光密度扫描。在腺体刺激下，腮腺唾液中组蛋白浓度（微克/毫升）无显著增加；然而，组蛋白分泌量（微克/分钟）增加了26倍（p<0.005；方差分析）。刺激下颌下/舌下唾液导致组蛋白浓度（p<0.005）和分泌量（p<0.0005）均显著增加。在从80名个体（分为四个年龄组，男女数量大致相等：35 - 44岁；45 - 54岁；55 - 64岁和65 - 76岁）收集的柠檬酸（0.1M）刺激的腮腺和下颌下/舌下唾液样本中测定年龄对唾液组蛋白的影响。所有患者均未服用药物或佩戴假牙。方差分析显示组蛋白在性别上无差异。回归分析显示，腮腺唾液组蛋白浓度（p<0.002）和分泌量（p<0.002）以及下颌下/舌下唾液组蛋白浓度（p<0.0001）和分泌量（p<0.0001）均与年龄显著相关下降。两种唾液类型每毫克总蛋白中的组蛋白浓度均显著下降（p<0.0001），这表明与总唾液蛋白相比，唾液组蛋白优先减少。这些结果表明，口腔宿主防御系统的唾液组蛋白成分会随着年龄增长而受损。
