Turnbull-Ross A D, Reavy B, Mayo M A, Murant A F
Scottish Crop Research Institute, Invergowrie, Dundee, U.K.
J Gen Virol. 1992 Dec;73 ( Pt 12):3203-11. doi: 10.1099/0022-1317-73-12-3203.
The complete sequence of 9871 nucleotides (nts) of parsnip yellow fleck virus (PYFV; isolate P-121) was determined from cDNA clones and by direct sequencing of viral RNA. The RNA contains a large open reading frame between nts 279 and 9362 which encodes a polyprotein of 3027 amino acids with a calculated M(r) of 336212 (336K). A PYFV polyclonal antiserum reacted with the proteins expressed from phage carrying cDNA clones from the 5' half of the PYFV genome. Comparison of the polyprotein sequence of PYFV with other viral polyprotein sequences reveals similarities to the putative NTP-binding and RNA polymerase domains of cowpea mosaic comovirus, tomato black ring nepovirus and several animal picornaviruses. The 3' untranslated region of PYFV RNA is 509 nts long and does not have a poly(A) tail. The 3'-terminal 121 nts may form a stem-loop structure which resembles that formed in the genomic RNA of mosquito-borne flaviviruses.
通过cDNA克隆和病毒RNA直接测序，确定了欧洲防风黄点病毒（PYFV；分离株P - 121）9871个核苷酸（nts）的完整序列。该RNA在核苷酸279至9362之间包含一个大的开放阅读框，其编码一个由3027个氨基酸组成的多聚蛋白，计算的分子量（M(r)）为336212（336K）。一种PYFV多克隆抗血清与携带PYFV基因组5' 半部分cDNA克隆的噬菌体所表达的蛋白质发生反应。将PYFV的多聚蛋白序列与其他病毒多聚蛋白序列进行比较，发现其与豇豆花叶病毒、番茄黑环病毒和几种动物微小核糖核酸病毒的假定NTP结合和RNA聚合酶结构域具有相似性。PYFV RNA的3' 非翻译区长度为509个核苷酸，且没有聚（A）尾。3' 末端的121个核苷酸可能形成一种茎环结构，类似于蚊媒黄病毒基因组RNA中形成的结构。
