葡萄扇叶病毒F13株系卫星RNA的核苷酸序列。

The nucleotide sequence of satellite RNA in grapevine fanleaf virus, strain F13.

作者信息

Fuchs M, Pinck M, Serghini M A, Ravelonandro M, Walter B, Pinck L

机构信息

Institut de Biologie Moléculaire des Plantes du C.N.R.S., Laboratoire de Virologie, Strasbourg, France.

出版信息

J Gen Virol. 1989 Apr;70 ( Pt 4):955-62. doi: 10.1099/0022-1317-70-4-955.

DOI:10.1099/0022-1317-70-4-955

PMID:2471799

Abstract

The nucleotide sequence of cDNA copies of grapevine fanleaf virus (strain F13) satellite RNA has been determined. The primary structure obtained was 1114 nucleotides in length, excluding the poly(A) tail, and contained only one long open reading frame encoding a 341 residue, highly hydrophilic polypeptide of Mr37275. The coding sequence was bordered by a leader of 14 nucleotides and a 3'-terminal non-coding region of 74 nucleotides. No homology has been found with small satellite RNAs associated with other nepoviruses. Two limited homologies of eight nucleotides have been detected between the satellite RNA in grapevine fanleaf virus and those in tomato black ring virus, and a consensus sequence U.G/UGAAAAU/AU/AU/A at the 5' end of nepovirus RNAs is reported. A less extended consensus exists in this region in comovirus and picornavirus RNA.

摘要

已确定葡萄扇叶病毒（F13株）卫星RNA的互补DNA（cDNA）拷贝的核苷酸序列。所获得的一级结构长度为1114个核苷酸，不包括聚腺苷酸（polyA）尾，并且仅包含一个长开放阅读框，编码一个由341个残基组成、Mr为37275的高度亲水性多肽。编码序列由14个核苷酸的前导序列和74个核苷酸的3'末端非编码区界定。未发现与其他线虫传多面体病毒相关的小卫星RNA有同源性。在葡萄扇叶病毒的卫星RNA与番茄黑环病毒的卫星RNA之间检测到两个8个核苷酸的有限同源性，并且报道了线虫传多面体病毒RNA 5'端的共有序列U.G/UGAAAAU/AU/AU/A。在豇豆花叶病毒和小RNA病毒RNA的该区域存在不太广泛的共有序列。