Connors Bret A, Evan Andrew P, Willis Lynn R, Simon Jay R, Fineberg Naomi S, Lifshitz David A, Shalhav Arieh L, Paterson Ryan F, Kuo Ramsay L, Lingeman James E
Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, Ind 46202, USA.
Nephron Physiol. 2003;95(4):p67-75. doi: 10.1159/000074843.
Renal blood flow falls in both kidneys following delivery of a clinical dose of shockwaves (SW) (2000 SW, 24 kV, Dornier HM3) to only one kidney. The role of renal nerves in this response was examined in a porcine model of renal denervation. Six-week-old pigs underwent unilateral renal denervation. Nerves along the renal artery of one kidney were identified, sectioned and painted with 10% phenol. Two weeks later the pigs were anesthetized and baseline renal function was determined using inulin and PAH clearances. Animals then had either sham-shockwave lithotripsy (SWL) (group 1), SWL to the innervated kidney (group 2) or SWL to the denervated kidney (group 3). Bilateral renal function was again measured 1 and 4 h after SWL. Both kidneys were then removed for analysis of norepinephrine content to validate the denervation. Renal plasma (RPF) flow was significantly reduced in shocked innervated kidneys (group 2) and shocked denervated kidneys (group 3). RPF was not reduced in the unshocked denervated kidneys of group 2. These observations suggest that renal nerves play a pivotal role in modulating the vascular response of the contralateral unshocked kidney to SWL, but only a partial role, if any, in modulating that response in the shocked kidney.
在仅对一侧肾脏给予临床剂量的冲击波（SW）（2000次SW，24 kV，多尼尔HM3型）后，两侧肾脏的肾血流量均会下降。在猪肾去神经支配模型中研究了肾神经在这种反应中的作用。六周龄的猪接受单侧肾去神经支配。识别一侧肾脏肾动脉周围的神经，切断并涂抹10%的苯酚。两周后，将猪麻醉，使用菊粉和对氨基马尿酸清除率测定基线肾功能。然后，动物分别接受假冲击波碎石术（SWL）（第1组）、对受神经支配的肾脏进行SWL（第2组）或对去神经支配的肾脏进行SWL（第3组）。在SWL后1小时和4小时再次测量双侧肾功能。然后取出双侧肾脏分析去甲肾上腺素含量以验证去神经支配效果。受冲击的受神经支配肾脏（第2组）和受冲击的去神经支配肾脏（第3组）的肾血浆流量（RPF）显著降低。第2组未受冲击的去神经支配肾脏的RPF未降低。这些观察结果表明，肾神经在调节对侧未受冲击的肾脏对SWL的血管反应中起关键作用，但在调节受冲击肾脏的这种反应中仅起部分作用（如果有作用的话）。
