Cairns N J, Chadwick A, Luthert P J, Lantos P L
Department of Neuropathology, Institute of Psychiatry, London, UK.
J Neurol Sci. 1992 Oct;112(1-2):68-75. doi: 10.1016/0022-510x(92)90134-7.
Alzheimer's disease (AD) temporal cortex (Brodmann area 22) was investigated using stains for astrocytes (GFAP immunohistochemistry), paired helical filaments (Gallyas silver impregnation) and beta A4-protein deposition (beta A4-protein immunohistochemistry). Paired helical filament formation (PHF), as demonstrated by neurofibrillary tangle (NFT) and neuritic plaque (NP) density, was greatest in the pyramidal cell layers III and V. beta A4-protein deposition was greatest in layer III but was present in all neocortical layers. In a regression analysis, astrocyte density was significantly correlated with beta A4-protein deposition (R2 = 0.35, P = 0.02). Astrocyte density was also positively correlated with PHF formation as measured by NFT (R2 = 0.16, P = 0.14) and NP (R2 = 0.25, P = 0.06) density, but this was less significant. This quantitative study demonstrates that both beta A4-protein deposits and PHF formation are positively correlated with the severity of astrocytosis and that damage to the brain parenchyma in temporal cortex in AD may be slightly more strongly associated with beta A4-protein deposition than paired helical filament formation. These results demonstrate the close association of astrocytes with beta A4-protein deposition and neuritic change in AD.
利用星形胶质细胞染色（胶质纤维酸性蛋白免疫组织化学法）、双螺旋丝染色（加利亚斯银浸染法）和β淀粉样蛋白沉积染色（β淀粉样蛋白免疫组织化学法）对阿尔茨海默病（AD）的颞叶皮质（布罗德曼22区）进行了研究。由神经原纤维缠结（NFT）和神经炎性斑块（NP）密度所显示的双螺旋丝形成（PHF）在Ⅲ层和Ⅴ层锥体细胞层中最为显著。β淀粉样蛋白沉积在Ⅲ层最为显著，但在所有新皮质层均有出现。在回归分析中，星形胶质细胞密度与β淀粉样蛋白沉积显著相关（R2 = 0.35，P = 0.02）。星形胶质细胞密度也与通过NFT（R2 = 0.16，P = 0.14）和NP（R2 = 0.25，P = 0.06）密度测量的PHF形成呈正相关，但显著性稍低。这项定量研究表明，β淀粉样蛋白沉积和PHF形成均与星形胶质细胞增生的严重程度呈正相关，并且AD中颞叶皮质脑实质的损伤可能与β淀粉样蛋白沉积的关联比与双螺旋丝形成的关联稍强。这些结果证明了星形胶质细胞与AD中β淀粉样蛋白沉积和神经炎性变化的密切关联。
