Armstrong R A, Myers D, Smith C U, Cairns N, Luthert P J
Clinical Neurophysiology Unit, Aston University, Birmingham, U.K.
Neurosci Lett. 1991 Feb 25;123(2):141-3. doi: 10.1016/0304-3940(91)90915-g.
The numerical density of senile plaques (SP) and neurofibrillary tangles (NFT) as revealed by the Glees silver method was compared with SP and NFT revealed by the Gallyas method and with amyloid (A4) deposits in immunostained sections in 6 elderly cases of Alzheimer's disease. The density of NFT was generally greater and A4 lower in tissue from hippocampus compared with the neocortex suggesting that A4 deposition was less important than the degree of paired helical filament (PHF) related damage in the hippocampus. The density of Glees SP was positively correlated Gallyas SP weakly correlated with A4 deposit number. A stepwise multiple regression analysis which included A4 deposit and Gallyas SP density and accounted for 54% of the variation in Glees SP density. Hence, different populations of SP were revealed by the different staining methods. The results suggested that the Glees method may stain a population of SP in a region of cortex where both amyloid deposition and neurofibrillary changes have occurred.
采用格利斯银染法显示的老年斑（SP）和神经原纤维缠结（NFT）的数值密度，与采用加利亚斯法显示的SP和NFT以及6例老年阿尔茨海默病患者免疫染色切片中的淀粉样蛋白（A4）沉积物进行了比较。与新皮质相比，海马组织中NFT的密度通常更高，而A4的密度更低，这表明在海马中，A4沉积不如成对螺旋丝（PHF）相关损伤程度重要。格利斯SP的密度与加利亚斯SP呈正相关，与A4沉积物数量呈弱相关。逐步多元回归分析纳入了A4沉积物和加利亚斯SP密度，解释了格利斯SP密度变化的54%。因此，不同的染色方法显示出不同的SP群体。结果表明，格利斯法可能在淀粉样蛋白沉积和神经原纤维变化均已发生的皮质区域对一群SP进行染色。
