Wan Weiguo, Xu Mailing, Zou Hejian, Lu Ailing, Shen Xinyu, Chen Yuming
Occupational Department, Huashan Hospital, Fudan University, Shanghai 200040, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2002 Dec;20(6):416-8.
To determine whether and to what degree the activity of cholinesterase(ChE) is inhibited by dimehypo at different doses of dimehypo [scientific name: 2-dimethylamine-1,3-bi(sodium hyposulfit)].
Rats were dosed with dimehypo or methamidophos orally, and were randomly divided into four subgroups according to the pesticide doses, which were 1/16, 1/8, 1/4 and 1/2 of LD50 respectively(the LD50 of dimethypo and methamidophos is 342 mg/kg and 20 mg/kg respectively). The activity of ChE in blood was determined before and 30 min, 1, 2, 4 and 24 h after exposure. The modified Ellman Method was employed to measure the activity of ChE.
1/16 LD50 dose of dimehypo did not affect the activity of ChE. When the dose increased, the activity of ChE decreased accordingly. 1/2 LD50 dose of dimehypo inhibited the activity of ChE by 35.9% compared with that of control group(P < 0.01). In rats dosed with methamidophos, even 1/16 LD50 dose inhibited the activity of ChE by 42.4% compared with that of control group. When the dose of methamidophos increased, the activity of ChE decreased accordingly. 1/2 LD50 dose of methamidophos inhibited the activity of ChE by 52.9%. The activity of ChE in the rats dosed with dimehypo at various doses was significantly lower than that in the rats dosed with corresponding doses of methamidophos(P < 0.01).
Higher doses of dimehypo may inhibit the activity of ChE. However, as compared with methamidophos, dimehypo is a weaker inhibitor of ChE.
确定不同剂量的敌百虫（学名：2 - 二甲胺基 - 1,3 - 双（亚硫酸氢钠））是否以及在何种程度上抑制胆碱酯酶（ChE）的活性。
给大鼠口服敌百虫或甲胺磷，并根据农药剂量将其随机分为四个亚组，剂量分别为半数致死量（LD50）的1/16、1/8、1/4和1/2（敌百虫和甲胺磷的LD50分别为342 mg/kg和20 mg/kg）。在暴露前以及暴露后30分钟、1小时、2小时、4小时和24小时测定血液中ChE的活性。采用改良的Ellman法测定ChE的活性。
1/16 LD50剂量的敌百虫对ChE的活性没有影响。当剂量增加时，ChE的活性相应降低。与对照组相比，1/2 LD50剂量的敌百虫使ChE的活性抑制了35.9%（P < 0.01）。在给予甲胺磷的大鼠中，即使是1/16 LD50剂量，与对照组相比也使ChE的活性抑制了42.4%。当甲胺磷剂量增加时，ChE的活性相应降低。1/2 LD50剂量的甲胺磷使ChE的活性抑制了52.9%。不同剂量敌百虫处理的大鼠中ChE的活性显著低于相应剂量甲胺磷处理的大鼠（P < 0.01）。
较高剂量的敌百虫可能抑制ChE的活性。然而，与甲胺磷相比，敌百虫是一种较弱的ChE抑制剂。
