Nagayama M, Akahori F, Chiwata H, Shirai M, Motoya M, Masaoka T, Sakaguchi K
Department of Veterinary Pharmacology, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Vet Hum Toxicol. 1996 Jun;38(3):196-9.
The serum cholinesterase (ChE) isoenzyme in rats shows 6 bands after polyacrylamide gradient gel electrophoresis. The effects of organophosphates (fenthion, chlorpyrifos, diazinon, bromophos, propaphos, haloxon, and DFP) on serum ChE isoenzyme bands were studied in 32 male and 32 female 6-w-old Sprague-Dawley rats. Each organophosphate was randomly administered to 4 male and 4 female rats. Blood samples were collected from the abdominal aorta under halothane anesthesia 6 h after dosing. The isoenzyme patterns were determined simultaneously with erythrocyte and serum ChE activities. Changes were observed in all 6 bands of the serum ChE isoenzymes after administration of fenthion, chlorpyrifos and propaphos. Diazinon had no influence on band 6, and DFP and bromophos had no influence on band 5. Haloxon did not effect any of the serum ChE isoenzyme bands. Serum ChE was most suppressed by fenthion, followed by DFP, bromophos, chlorpyrifos, propaphos and diazinon in that order of effect. Serum ChE activity was not suppressed by haloxon. Erythrocyte ChE activity was suppressed by every organophosphate. This experiment demonstrated a correlation between the organophosphate suppression of serum ChE activity and the concentration of serum ChE isoenzyme band 6.
大鼠血清胆碱酯酶（ChE）同工酶在聚丙烯酰胺梯度凝胶电泳后显示出6条带。在32只6周龄的雄性和32只雌性Sprague-Dawley大鼠中研究了有机磷酸酯（倍硫磷、毒死蜱、二嗪农、溴磷、丙溴磷、氧硫磷和二异丙基氟磷酸酯）对血清ChE同工酶带的影响。每种有机磷酸酯随机给予4只雄性和4只雌性大鼠。给药6小时后，在氟烷麻醉下从腹主动脉采集血样。同时测定同工酶模式以及红细胞和血清ChE活性。给予倍硫磷、毒死蜱和丙溴磷后，血清ChE同工酶的所有6条带均出现变化。二嗪农对第6条带无影响，二异丙基氟磷酸酯和溴磷对第5条带无影响。氧硫磷对任何血清ChE同工酶带均无影响。血清ChE受倍硫磷抑制作用最强，其次依次为二异丙基氟磷酸酯、溴磷、毒死蜱、丙溴磷和二嗪农。氧硫磷未抑制血清ChE活性。每种有机磷酸酯均抑制红细胞ChE活性。本实验证明了有机磷酸酯对血清ChE活性的抑制作用与血清ChE同工酶第6条带浓度之间的相关性。
