[肝细胞癌组织中碱性磷酸酶糖链的变化]

[Changes of alkaline phosphatase sugar chains in hepatocellular carcinoma tissue].

作者信息

Chen Guo-qian, Zhang Qing, Xu Yan-fang, Zhang Wan-zhong, Guan Ming, Su Bing, Liang Hui-qi, Lu Yuan

机构信息

Department of Clinical Laboratory, Hua Shan Hospital, Shanghai 200040, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2003 Dec;11(12):739-41.

PMID:14697136

Abstract

OBJECTIVE

To investigate the changes of sugar chain structures of alkaline phosphatase (ALP) in hepatoma tissue and its relation to the invasiveness of hepatocellular carcinoma (HCC).

METHODS

The binding ratios of ALP from 9 normal liver tissues, 16 hepatoma tissues and 16 noncancerous tissues surrounding hepatoma were analysed by affinity chromatography on various lectin columns including leukoagglutinating phytohemagglutinin (L-PHA), lentil lectin (LCA), Datura stramonium agglutinin (DSA), erythroagglutinating phytohemagglutinin (E-PHA) and Sambucus nigra bark agglutinin (SNA).

RESULTS

The binding ratios of ALP on L-PHA (22.94%+/-5.30%), DSA (55.97%+/-13.72%), LCA (38.16%+/-8.87%), E-PHA (11.56%+/-4.81%) and SNA (69.80%+/-13.71%) in HCC tissues were significantly increased (P<0.01) compared with that in normal liver tissues (L-PHA 5.89%+/-2.75%, DSA 36.20%+/-11.58%, LCA 17.90%+/-6.71%, E-PHA 5.38%+/-2.20%, SNA 57.32%+/-11.27%), respectively. t values between the two groups were 8.94, 3.64, 5.94, 3.62 and 2.32, respectively. L-PHA-binding ratio (25.84%+/-4.67%) of ALP in HCC with invasiveness was significantly higher than that (18.10%+/-3.64%) without invasiveness (t=3.71, P<0.01).

CONCLUSION

The changes of ALP sugar chain structures occur in HCC tissue. b1-6 branching sugar chain structure of ALP is related to the invasiveness of HCC.

摘要

目的

研究肝癌组织中碱性磷酸酶（ALP）糖链结构的变化及其与肝细胞癌（HCC）侵袭性的关系。

方法

采用包括白细胞凝集素（L-PHA）、扁豆凝集素（LCA）、曼陀罗凝集素（DSA）、红细胞凝集素（E-PHA）和接骨木树皮凝集素（SNA）等多种凝集素柱亲和层析法，分析9例正常肝组织、16例肝癌组织及16例肝癌旁非癌组织中ALP的结合率。

结果

与正常肝组织（L-PHA 5.89%±2.75%，DSA 36.20%±11.58%，LCA 17.90%±6.71%，E-PHA 5.38%±2.20%，SNA 57.32%±11.27%）相比，肝癌组织中ALP在L-PHA（22.94%±5.30%）、DSA（55.97%±13.72%）、LCA（38.16%±8.87%）、E-PHA（11.56%±4.81%）和SNA（69.80%±13.71%）上的结合率均显著升高（P<0.01）。两组间的t值分别为8.94、3.64、5.94、3.62和2.32。有侵袭性的肝癌组织中ALP的L-PHA结合率（25.84%±4.67%）显著高于无侵袭性的肝癌组织（18.10%±3.64%）（t=3.71，P<0.01）。