Saitoh A, Aoyagi Y, Asakura H
Department of Internal Medicine, Niigata University School of Medicine, Japan.
Arch Biochem Biophys. 1993 Jun;303(2):281-7. doi: 10.1006/abbi.1993.1284.
Chemical structures of the sugar chains of alpha 1-antitrypsin (AAT) from patients with hepatocellular carcinoma (HCC) and from healthy individuals with a different affinity for Lens culinaris agglutinin (LCA) were examined by pyridylamination of their oligosaccharides and stepwise exoglycosidase digestion in combination with reversed-phase and size-fractionation high-performance liquid chromatography. We found that the LCA-reactive species of AAT from patients with HCC carried both the biantennary sugar chain with a fucose residue at the innermost N-acetylglucosamine residue, Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6(Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc-PA, and the biantennary chain without a fucose residue, Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6(Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4GlcNAc-PA, at a ratio of about 1:0.6. The LCA-nonreactive species of AAT contained the biantennary sugar chain Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6(Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4GlcNAc as a major component. These results indicate that a characteristic feature of the carbohydrate chains of AAT from patients with HCC is an increment in fucosylation.
通过对来自肝细胞癌（HCC）患者和对豆凝集素（LCA）具有不同亲和力的健康个体的α1-抗胰蛋白酶（AAT）糖链进行吡啶基化寡糖分析，并结合反相和尺寸分级高效液相色谱进行逐步外切糖苷酶消化，研究了其化学结构。我们发现，HCC患者的AAT中与LCA反应的物种同时携带在最内侧N-乙酰葡糖胺残基处带有岩藻糖残基的双天线糖链，即Galβ1-4GlcNAcβ1-2Manα1-6（Galβ1-4GlcNAcβ1-2Manα1-3）Manβ1-4GlcNAcβ1-4（Fucα1-6）GlcNAc-PA，以及不含岩藻糖残基的双天线链，即Galβ1-4GlcNAcβ1-2Manα1-6（Galβ1-4GlcNAcβ1-2Manα1-3）Manβ1-4GlcNAcβ1-4GlcNAc-PA，比例约为1:0.6。AAT中与LCA不反应的物种主要包含双天线糖链Galβ1-4GlcNAcβ1-2Manα1-6（Galβ1-4GlcNAcβ1-2Manα1-3）Manβ1-4GlcNAcβ1-4GlcNAc。这些结果表明，HCC患者AAT糖链的一个特征是岩藻糖基化增加。
