Si Kausik, Giustetto Maurizio, Etkin Amit, Hsu Ruby, Janisiewicz Agnieszka M, Miniaci Maria Conchetta, Kim Joung-Hun, Zhu Huixiang, Kandel Eric R
Center for Neurobiology and Behavior, College of Physicians and Surgeons of Columbia University, New York State Psychiatric Institute, 722 West 168th Street, New York, NY 10032, USA.
Cell. 2003 Dec 26;115(7):893-904. doi: 10.1016/s0092-8674(03)01021-3.
Synapse-specific facilitation requires rapamycin-dependent local protein synthesis at the activated synapse. In Aplysia, rapamycin-dependent local protein synthesis serves two functions: (1) it provides a component of the mark at the activated synapse and thereby confers synapse specificity and (2) it stabilizes the synaptic growth associated with long-term facilitation. Here we report that a neuron-specific isoform of cytoplasmic polyadenylation element binding protein (CPEB) regulates this synaptic protein synthesis in an activity-dependent manner. Aplysia CPEB protein is upregulated locally at activated synapses, and it is needed not for the initiation but for the stable maintenance of long-term facilitation. We suggest that Aplysia CPEB is one of the stabilizing components of the synaptic mark.
突触特异性易化需要在激活的突触处进行雷帕霉素依赖性的局部蛋白质合成。在海兔中，雷帕霉素依赖性的局部蛋白质合成发挥两种功能：（1）它在激活的突触处提供标记的一个组成部分，从而赋予突触特异性；（2）它稳定与长期易化相关的突触生长。在此我们报告，细胞质聚腺苷酸化元件结合蛋白（CPEB）的一种神经元特异性同工型以活性依赖的方式调节这种突触蛋白质合成。海兔CPEB蛋白在激活的突触处局部上调，并且它不是长期易化起始所必需的，而是长期易化稳定维持所必需的。我们认为海兔CPEB是突触标记的稳定成分之一。
