Balasundaresan D, Saraboji K, Ponnuswamy M N
Department of Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600025, India.
Biochem Biophys Res Commun. 2004 Jan 16;313(3):466-7. doi: 10.1016/j.bbrc.2003.11.137.
Haemoglobin acts as an important protein for oxygen carrier in all living beings. Purification of donkey haemoglobin was done using PEG 4000 as a separating medium. Crystallization was achieved using hanging drop vapor diffusion method using 2.8 M phosphate buffer, pH 6.8. Data collection was done using mar345 image plate detector system. The crystals grown under 2.8 M phosphate buffer are monoclinic with space group C2 and cell dimensions a=107.664 A, b=63.084 A, c=54.042 A, and beta=111.747 degrees.
血红蛋白是所有生物中重要的氧载体蛋白。使用聚乙二醇4000作为分离介质对驴血红蛋白进行了纯化。采用悬滴气相扩散法，使用pH 6.8的2.8 M磷酸盐缓冲液实现了结晶。使用mar345成像板探测器系统进行数据收集。在2.8 M磷酸盐缓冲液中生长的晶体为单斜晶系，空间群为C2，晶胞参数a = 107.664 Å，b = 63.084 Å，c = 54.042 Å，β = 111.747°。
