Balasundaresan D, Saraboji K, Ponnuswamy M N
Department of Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, 600025 India.
Biochimie. 2006 Jun;88(6):719-23. doi: 10.1016/j.biochi.2006.01.001. Epub 2006 Jan 26.
Haemoglobin from donkey was purified and crystallized in space group C2. The present donkey haemoglobin model comprises of two subunits alpha and beta. These alpha and beta subunits comprise of 141 and 146 amino acid residues, respectively, and the haem groups. The donkey haemoglobin differs from horse only in two amino acids of alpha-chain (His20 to Asn and Tyr24 to Phe) and these substitutions do not significantly change the secondary structural features of donkey haemoglobin. The haem group region and subunit contacts are closely resemble with that of horse methaemoglobin.
驴血红蛋白在空间群C2中被纯化并结晶。目前的驴血红蛋白模型由两个亚基α和β组成。这些α和β亚基分别由141个和146个氨基酸残基以及血红素基团组成。驴血红蛋白与马血红蛋白的区别仅在于α链的两个氨基酸（His20变为Asn，Tyr24变为Phe），并且这些取代并没有显著改变驴血红蛋白的二级结构特征。血红素基团区域和亚基接触与马高铁血红蛋白的非常相似。
