González-Aguilar G A, Tiznado-Hernández M E, Zavaleta-Gatica R, Martínez-Téllez M A
Centro de Investigación en Alimentación y Desarrollo, AC, Coordinación de Tecnología de Alimentos de Origen Vegetal, Carretera a la Victoria Km 0.6, A P 1735, Hermosillo 83000, Sonora, Mexico.
Biochem Biophys Res Commun. 2004 Jan 16;313(3):694-701. doi: 10.1016/j.bbrc.2003.11.165.
Tropical fruits cannot be stored at low temperatures due to the chilling injury phenomena. With the goal of reducing the chilling injury, we tested 10(-4) and 10(-5) M of methyl jasmonate (MJ) treatment before the storage of red and white cultivars of guava fruits at 5 degrees C for up to 15 days plus two days at 20 degrees C. Every five days, we evaluated chilling injury index, ion leakage percentage, vitamin C, sugars, total phenols, and the activity of the enzymes lipoxygenase (LOX) and phenylalanine-ammonia lyase (PAL). We found that methyl jasmonate treatments reduce the chilling injury index and the ion leakage percentage. Furthermore, MJ did not affect vitamin C, chlorophyll, and total phenols. MJ increased sugar content, PAL, and LOX activities. We concluded that MJ reduces chilling injury and activates the fruit defense response as indicated by the behavior of total phenols and the increase in sugar content, PAL, and LOX activities.
由于冷害现象，热带水果不能在低温下储存。为了减少冷害，我们在将番石榴果实的红色和白色品种在5℃下储存长达15天并在20℃下储存两天之前，测试了10(-4)和10(-5)M的茉莉酸甲酯（MJ）处理。每五天，我们评估冷害指数、离子渗漏百分比、维生素C、糖分、总酚以及脂氧合酶（LOX）和苯丙氨酸解氨酶（PAL）的酶活性。我们发现茉莉酸甲酯处理降低了冷害指数和离子渗漏百分比。此外，MJ不影响维生素C、叶绿素和总酚。MJ增加了糖分含量、PAL和LOX活性。我们得出结论，MJ减少了冷害并激活了果实防御反应，这表现为总酚的变化以及糖分含量、PAL和LOX活性的增加。
