Fujita Toshitsugu, Ikuta Junko, Hamada Juri, Okajima Toshihide, Tatematsu Kenji, Tanizawa Katsuyuki, Kuroda Shun'ichi
Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567-0047, Japan.
Biochem Biophys Res Commun. 2004 Jan 16;313(3):738-44. doi: 10.1016/j.bbrc.2003.12.006.
Fasciculation and elongation protein zeta-1 (FEZ1) is a mammalian orthologue of the Caenorhabditis elegans UNC-76 protein involved in the axonal outgrowth and fasciculation and promotes neurite extension of PC12 cells through interaction with protein kinase C zeta (PKCzeta). The gene coding for FEZ2, a homologue of FEZ1, has also been reported in rat and human. In this study, we compared mRNA expression of FEZ1 and FEZ2 in adult rat tissues and mouse embryos by Northern blot and in situ hybridization analyses. In contrast to FEZ1 whose mRNA is expressed almost exclusively in rat brain and temporarily around the neurogenesis stage of mouse embryos, the message for FEZ2 is detected weakly in most tissues and abundantly throughout the mouse embryonic stages. Similar to FEZ1, FEZ2 interacted with PKCzeta and induced neurite extension of PC12 cells when coexpressed with a constitutively active mutant of PKCzeta. These results suggest that FEZ2 plays an important role in the morphological changes of various cells by associating with PKCzeta in a tissue-non-specific manner.
成束和延伸蛋白ζ-1（FEZ1）是秀丽隐杆线虫UNC-76蛋白的哺乳动物同源物，参与轴突生长和成束，并通过与蛋白激酶Cζ（PKCζ）相互作用促进PC12细胞的神经突延伸。编码FEZ1同源物FEZ2的基因也已在大鼠和人类中报道。在本研究中，我们通过Northern印迹和原位杂交分析比较了FEZ1和FEZ2在成年大鼠组织和小鼠胚胎中的mRNA表达。与FEZ1不同，FEZ1的mRNA几乎只在大鼠脑中表达，并在小鼠胚胎神经发生阶段暂时表达，而FEZ2的信息在大多数组织中微弱检测到，并且在整个小鼠胚胎阶段大量表达。与FEZ1相似，FEZ2与PKCζ相互作用，并在与PKCζ的组成型活性突变体共表达时诱导PC12细胞的神经突延伸。这些结果表明，FEZ2通过以组织非特异性方式与PKCζ结合，在各种细胞的形态变化中起重要作用。
