Yamamoto Toshiharu, Suzuki Hirohumi, Uemura Haruko, Yamamoto Kazutoshi, Kikuyama Sakaé
Department of Biology, Kanagawa Dental College, Inaoka-cho 82, Yokosuka, Kanagawa 238-8580, Japan.
Gen Comp Endocrinol. 2004 Jan 15;135(2):186-92. doi: 10.1016/j.ygcen.2003.09.004.
Immunohistochemical techniques were employed to investigate the distribution of orexin-A-like immunoreactivity in the bullfrog (Rana catesbeiana) pituitary. Orexin-A-immunoreactive cells were scattered throughout the pars distalis. We found that these cells corresponded to the cells immunostained with antiserum against bullfrog prolactin (fPRL). Immunoelectron microscopic analysis indicated that an orexin-A-like substance coexisted with fPRL within secretory granules. Western blot analysis of bullfrog pituitary extract revealed that anti-human orexin-A antiserum labeled two separate bands which were not labeled with anti-fPRL antiserum. The present study has, for the first time, provided evidence of the intragranular colocalization of orexin-A-like and PRL immunoreactivities in the bullfrog pituitary.
采用免疫组织化学技术研究牛蛙（Rana catesbeiana）垂体中食欲素A样免疫反应性的分布。食欲素A免疫反应性细胞散布于远侧部。我们发现这些细胞与用抗牛蛙催乳素（fPRL）抗血清免疫染色的细胞相对应。免疫电子显微镜分析表明，一种食欲素A样物质与fPRL共存于分泌颗粒内。对牛蛙垂体提取物的蛋白质免疫印迹分析显示，抗人食欲素A抗血清标记出两条单独的条带，而抗fPRL抗血清未标记这两条带。本研究首次提供了牛蛙垂体中食欲素A样和PRL免疫反应性在颗粒内共定位的证据。
