Suzuki H, Takemoto Y, Yamamoto T
Department of Biology, Fukuoka University of Education, Akamabunkyo-machi 1-1, Munakata, Fukuoka 811-4192, Japan.
Tissue Cell. 2007 Dec;39(6):423-30. doi: 10.1016/j.tice.2007.08.001. Epub 2007 Sep 25.
Immunohistochemical techniques were employed to investigate orexin-A-like and orexin receptor 1 (OX1R)-like immunoreactivities in the Xenopus pituitary gland. Orexin-A-immunoreactive cells were mainly scattered in the posterior half of the pars distalis. They corresponded to thyroid-stimulating hormone (TSH)-containing cells and so far have not corresponded to other types of pituitary adenocytes. On the other hand, OX1R-immunoreactive cells were mainly distributed in the anterior half of the pars distalis and corresponded to prolactin (PRL)-containing cells; however, we found that OX1R-immunoreactive cells did not correspond to other types of adenocytes in the Xenopus pituitary. These results suggest that an orexin-A-like substance secretes with and/or without TSH from TSH-containing cells and that the peptide modulates the functions of PRL-containing cells via OX1R in a paracrine fashion.
采用免疫组织化学技术研究非洲爪蟾垂体中orexin - A样和orexin受体1（OX1R）样免疫反应性。orexin - A免疫反应性细胞主要散在于远侧部后半部。它们对应于含促甲状腺激素（TSH）的细胞，迄今为止尚未对应于其他类型的垂体腺细胞。另一方面，OX1R免疫反应性细胞主要分布在远侧部前半部，对应于含催乳素（PRL）的细胞；然而，我们发现非洲爪蟾垂体中的OX1R免疫反应性细胞与其他类型的腺细胞不对应。这些结果表明，orexin - A样物质从含TSH的细胞中与TSH一起分泌和/或不与TSH一起分泌，并且该肽以旁分泌方式通过OX1R调节含PRL细胞的功能。
