Mwaba Peter, Cassol Sharon, Nunn Andrew, Pilon Rick, Chintu Chifumbe, Janes Michelle, Zumla Alimuddin
University of Zambia-University College London Medical School (UNZA-UCLMS) Research and Training Project, University Teaching Hospital, Lusaka, Zambia.
Lancet. 2003 Dec 20;362(9401):2067-8. doi: 10.1016/S0140-6736(03)15103-3.
The increasing availability of antiretroviral drugs to HIV-infected populations in developing countries highlights the need to develop field-friendly practical methods for HIV-1 viral load measurements to monitor the effects of treatment. We compared use of whole-blood spots versus plasma dried on filter paper to quantify HIV-1 viral load in 51 African patients with HIV-1. The mean log10 HIV-1 viral loads were 4.22 for dried plasma spots (DPS) and 4.20 for dried whole-blood spots (DBS). The difference between the pairs of log10 viral load for DPS and DBS were significantly correlated with their mean (Spearman's r=0.31, p=0.03). This correlation between the difference and mean of viral load was no longer evident for values of log10 DPS that were less than 5 (r=0.01, p=0.93). For the 38 paired values with log10DPS of less than 5, the mean difference (log10DPS-log10DBS) was -0.04 (SD 0.29). Dried whole blood stored on filter paper at room temperature shows potential as a field-friendly alternative to plasma for measurement of HIV-1 viral load.
在发展中国家，抗逆转录病毒药物越来越容易获得，这凸显了开发便于在现场使用的实用方法来测量HIV-1病毒载量以监测治疗效果的必要性。我们比较了使用全血斑点与滤纸上干燥的血浆来定量51例HIV-1感染的非洲患者的HIV-1病毒载量。干燥血浆斑点（DPS）的平均log10 HIV-1病毒载量为4.22，干燥全血斑点（DBS）为4.20。DPS和DBS的log10病毒载量对之间的差异与其平均值显著相关（Spearman相关系数r = 0.31，p = 0.03）。对于log10 DPS小于5的值，病毒载量差异与平均值之间的这种相关性不再明显（r = 0.01，p = 0.93）。对于38对log10DPS小于5的配对值，平均差异（log10DPS - log10DBS）为-0.04（标准差0.29）。室温下储存在滤纸上的干燥全血显示出作为测量HIV-1病毒载量的血浆的现场友好替代物的潜力。
