Cucinotta Vincenzo, Giuffrida Alessandro, La Mendola Diego, Maccarrone Giuseppe, Puglisi Antonino, Rizzarelli Enrico, Vecchio Graziella
Dipartimento di Scienze Chimiche, University of Catania, V.le A. Doria 6, Catania 95125, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Feb 5;800(1-2):127-33. doi: 10.1016/j.jchromb.2003.08.040.
The systems that the 3-amino derivative of beta-cyclodextrin (CD3NH2) forms with the proton, the copper(II) ion and each of the enantiomers of certain amino acids (alanine, phenylalanine, tyrosine and tryptophan) were investigated. The enantioselectivity shown by the potentiometric measurements carried out on the phenylalanine ternary systems was exploited in capillary electrophoresis by ligand exchange capillary electrophoresis (LECE) to obtain the separation of phenylalanine racemate. The tyrosine racemate was also separated by LECE. The comparison between thermodynamic and capillary electrophoresis (CE) results is discussed, in order to get a better insight into the separation mechanism.
