Michalska Katarzyna, Pajchel Genowefa, Tyski Stefan
Antibiotics and Microbiology Department, National Institute of Public Health, 30-34 Chelmska Street, 00-725 Warsaw, Poland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Feb 5;800(1-2):203-9. doi: 10.1016/j.jchromb.2003.10.017.
Capillary electrophoresis method for identification and simultaneous determination of procaine, dihydrostreptomycin and penicillin G, present in multiantibiotic veterinary preparations, was elaborated. The influence of pH (5.0-9.75) and concentration of disodium tetraborate decahydrate in running buffers (0.02-0.1 M) as well as temperatures (25-40 degrees C) on separation efficacy were analyzed. For quantitative analysis, 0.08 M borate buffer (pH 8.0) at 35 degrees C and 15 kV were chosen. Method was validated, selectivity, precision, linearity, LOD, LOQ, accuracy and specificity of capillary zone electrophoresis (CZE) were evaluated.
