Avula B, Begum S, Ahmed S, Choudhary M I, Khan I A
National Center for Natural Products Research, University of Mississippi, MS 38677, USA.
Pharmazie. 2008 Jan;63(1):20-2.
A new method of capillary electrophoresis was developed for the quantitative determination of vasicine and vasicinone from Adhatoda vasica (L.) Nees. The electrophoretic separation was performed using a 47 cm x 50 microm ID (38.5 cm effective length) fused silica capillary. The samples were injected by pressure for 3 s at 50 mbar and the running voltage was 19 kV at the injector end of the capillary. The capillary temperature was maintained at 40 degrees C. The separation of the two alkaloids has been achieved within 11 min with good repeatability. The method was validated in terms of reproducibility, linearity, accuracy and applied for the quantitative determination of vasicine and vasicinone in A. vasica plant samples/extracts. Parameters affecting the resolution such as pH, temperature, organic modifier, buffer concentration and capillary dimensions were reported.
开发了一种新的毛细管电泳方法，用于定量测定鸭嘴花中的鸭嘴花碱和去甲鸭嘴花碱。使用内径为50μm（有效长度为38.5cm）、长度为47cm的熔融石英毛细管进行电泳分离。样品在50mbar压力下通过压力进样3s，毛细管进样端的运行电压为19kV。毛细管温度保持在40℃。两种生物碱在11min内实现了分离，重现性良好。该方法在重现性、线性、准确性方面得到了验证，并应用于鸭嘴花植物样品/提取物中鸭嘴花碱和去甲鸭嘴花碱的定量测定。报告了影响分离度的参数，如pH值、温度、有机改性剂、缓冲液浓度和毛细管尺寸。
