Bird Phillip I, Pak Stephen C, Worrall D Margaret, Bottomley Stephen P
Department of Biochemistry and Molecular Biology, Monash University 3800, Australia.
Methods. 2004 Feb;32(2):169-76. doi: 10.1016/s1046-2023(03)00208-1.
Expression systems based on Escherichia coli offer fast, cheap, and convenient means for the production of recombinant serpins. Over 30 active serpins from prokaryotic and eukaryotic organisms have been produced in this way, using a variety of vectors, promoters, fusion partners, and host strains. Serpins forming insoluble inclusion bodies in E. coli can generally be solubilized and refolded. Here, we outline the general approaches and procedures to be considered when contemplating the use of E. coli for recombinant serpin production.
基于大肠杆菌的表达系统为重组丝氨酸蛋白酶抑制剂的生产提供了快速、廉价且便捷的方法。利用多种载体、启动子、融合伴侣和宿主菌株，已通过这种方式生产出了30多种来自原核生物和真核生物的活性丝氨酸蛋白酶抑制剂。在大肠杆菌中形成不溶性包涵体的丝氨酸蛋白酶抑制剂通常可以溶解并复性。在此，我们概述了在考虑使用大肠杆菌进行重组丝氨酸蛋白酶抑制剂生产时应考虑的一般方法和步骤。
