在细菌表达系统中生产活性真核蛋白的策略。
Strategies for production of active eukaryotic proteins in bacterial expression system.
作者信息
Khow Orawan, Suntrarachun Sunutcha
机构信息
Queen Saovabha Memorial Institute, Thai Red Cross Society, Bangkok 10330, Thailand.
出版信息
Asian Pac J Trop Biomed. 2012 Feb;2(2):159-62. doi: 10.1016/S2221-1691(11)60213-X.
Abstract
Bacteria have long been the favorite expression system for recombinant protein production. However, the flaw of the system is that insoluble and inactive proteins are co-produced due to codon bias, protein folding, phosphorylation, glycosylation, mRNA stability and promoter strength. Factors are cited and the methods to convert to soluble and active proteins are described, for example a tight control of Escherichia coli milieu, refolding from inclusion body and through fusion technology.
摘要
长期以来，细菌一直是重组蛋白生产中最受欢迎的表达系统。然而，该系统的缺陷在于，由于密码子偏好、蛋白质折叠、磷酸化、糖基化、mRNA稳定性和启动子强度等因素，会同时产生不溶性和无活性的蛋白质。文中列举了相关因素，并描述了将其转化为可溶性和活性蛋白质的方法，例如严格控制大肠杆菌环境、从包涵体中重折叠以及通过融合技术。
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