Sedaghat M M, Linton Y-M, Oshaghi M A, Vatandoost H, Harbach R E
Department of Medical Entomology, School of Public Health, Tehran University of Medical Sciences, Iran.
Bull Entomol Res. 2003 Dec;93(6):527-35. doi: 10.1079/ber2003272.
Mosquitoes of the Anopheles maculipennis complex were collected in nine provinces of Iran (Esfahan, Fars, Gilan, Golestan, Kohkiluyeh va Boyerahmad, Mazandaran, Tehran, Azarbaijan-e Gharbi and Zanjan) between June 1983 and September 2002. The nuclear rDNA ITS2 sequences of 86 specimens were compared with those of seven species of the complex available in GenBank. Three genetically distinct species of the complex were distinguished: A. maculipennis Meigen, A. sacharovi Favre and a previously unrecognized species. The last species is most similar to, but clearly distinct from, A. martinius Shingarev and A. sacharovi. The taxonomy of A. martinius and A. sacharovi is critically reviewed, and justification is provided for formally recognizing the third species as Anopheles persiensis sp.n. The new species is the first culicid to be characterized and named principally on the basis of DNA evidence. Anopheles persiensis was collected only in the northern Caspian Sea littoral provinces of Gilan and Mazandaran, and it seems likely that this species could be responsible for malaria transmission in this region that was previously attributed to A. maculipennis. A species-specific RFLP-PCR assay based on ITS2 sequences was developed to facilitate further studies of the three species in Iran.
1983年6月至2002年9月期间，在伊朗的九个省份（伊斯法罕、法尔斯、吉兰、戈勒斯坦、科吉卢耶-博韦艾哈迈德、马赞德兰、德黑兰、西阿塞拜疆和赞詹）采集了黄斑按蚊复合体的蚊子。将86个样本的核rDNA ITS2序列与GenBank中该复合体的七个物种的序列进行了比较。区分出该复合体的三个遗传上不同的物种：梅氏按蚊、萨氏按蚊和一个以前未被识别的物种。最后一个物种与马丁按蚊和萨氏按蚊最为相似，但明显不同。对马丁按蚊和萨氏按蚊的分类进行了严格审查，并为正式将第三个物种确认为波斯按蚊提供了依据。这个新物种是第一个主要基于DNA证据进行特征描述和命名的蚊科物种。波斯按蚊仅在里海北部沿岸的吉兰和马赞德兰省份采集到，似乎该物种可能是该地区以前归因于梅氏按蚊的疟疾传播的原因。基于ITS2序列开发了一种物种特异性RFLP-PCR检测方法，以促进对伊朗这三个物种的进一步研究。
