马混合淋巴细胞培养的微量培养方法。
Microculture method for mixed lymphocyte cultures in the horse.
作者信息
McClure J J, Muscoplat C C, Johnson D W, Senogles D R
出版信息
Am J Vet Res. 1978 Feb;39(2):337-9.
PMID:147041
Abstract
A miniaturized method for the mixed lymphocyte culture test in the horse is described. The test is performed in either round- or flat-bottom microtitration tissue culture plates. Concentrations of responsing and stimulating cells are varied, depening on the experiment. Significant discrimination between isogeneic and allogenic mixtures is possible after 120 hours' culture when cells are labeled ([3H]thymidine) for the last 16 to 18 hours of the test.
摘要
本文描述了一种用于马的混合淋巴细胞培养试验的小型化方法。该试验在圆底或平底微量滴定组织培养板中进行。根据实验不同，反应细胞和刺激细胞的浓度会有所变化。当在试验的最后16至18小时对细胞进行标记（[3H]胸腺嘧啶核苷）时，培养120小时后，同基因和异基因混合物之间能够实现显著区分。
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