Bailey M, Lloyd S, Martin S C, Soulsby E J
Z Parasitenkd. 1984;70(2):229-42. doi: 10.1007/BF00942226.
Proliferation in vitro of peripheral blood lymphocytes both from horses infected with Strongylus vulgaris and from helminth-free ponies was observed in the presence of extracts of the fourth and fifth stage larvae and adults of S. vulgaris. In addition, S. vulgaris extracts induced transformation in cultures of peripheral blood lymphocytes from sheep and dogs and in mouse spleen cell cultures. Nylon wool non-adherent, T cell enriched fractions of lymphocytes from both mice and horses were stimulated by the S. vulgaris larval mitogen while no proliferation was observed in cultures containing nylon wool adherent, B cell enriched fractions. Macrophage co-operation appeared not to be necessary for S. vulgaris mitogen-induced transformation of spleen cells. The S. vulgaris mitogen stimulated a subpopulation of mouse spleen cells different from those responsive to PHA, Con A and LPS. These cells might be T helper cells since B cells were stimulated to proliferate in the presence of both T cells and S. vulgaris larval mitogen. In addition, the supernatant of in vitro cultured larvae of S. vulgaris induced slight, but significant transformation of equine peripheral blood lymphocytes. Therefore, it is possible that the S. vulgaris mitogen released by both viable parasites and degenerating larvae might induce T cell dependent production of immunoglobulin in vivo and account for the beta-globulinaemia, of which IgG(T) is a major component, in S vulgaris infected horses.
在存在普通圆线虫第四和第五期幼虫及成虫提取物的情况下，观察到感染普通圆线虫的马和未感染蠕虫的小马外周血淋巴细胞的体外增殖。此外，普通圆线虫提取物可诱导绵羊和狗的外周血淋巴细胞培养物以及小鼠脾细胞培养物发生转化。普通圆线虫幼虫促有丝分裂原可刺激来自小鼠和马的尼龙毛非黏附性、富含T细胞的淋巴细胞组分，而在含有尼龙毛黏附性、富含B细胞的组分的培养物中未观察到增殖现象。巨噬细胞协同作用似乎并非普通圆线虫促有丝分裂原诱导脾细胞转化所必需。普通圆线虫促有丝分裂原刺激的小鼠脾细胞亚群不同于对PHA、Con A和LPS有反应的细胞亚群。这些细胞可能是T辅助细胞，因为在T细胞和普通圆线虫幼虫促有丝分裂原存在的情况下，B细胞会被刺激增殖。此外，普通圆线虫体外培养幼虫的上清液可诱导马外周血淋巴细胞发生轻微但显著的转化。因此，活寄生虫和退化幼虫释放的普通圆线虫促有丝分裂原可能在体内诱导依赖T细胞的免疫球蛋白产生，并解释了感染普通圆线虫的马出现的β球蛋白血症，其中IgG(T)是主要成分。
