Sedohara Ayako, Komazaki Shinji, Asashima Makoto
Department of Life Sciences (Biology), Graduate school of Art and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, 153-8902, Japan.
Dev Growth Differ. 2003 Oct-Dec;45(5-6):463-71. doi: 10.1111/j.1440-169x.2003.00713.x.
A vertebrate eye was induced via a series of coordinated inductive interactions. Here, we describe a novel in vitro system to induce eye formation at high frequency using Xenopus early gastrulae. The eye formed in vitro is morphologically similar to the normal eye. When the in vitro eye was transplanted into a stage-33 tadpole, the optic nerve was seen extending from the grafted eye to the tectum of the host brain and the in vitro eye graft was retained after metamorphosis. In addition, we transplanted the eye formed in vitro into a tadpole with both eyes removed. The resultant juvenile frogs could perceive brightness using the grafted eye and thereby control their skin color, suggesting that the eye formed in vitro could function normally.
通过一系列协调的诱导相互作用诱导出了脊椎动物的眼睛。在此，我们描述了一种新的体外系统，该系统利用非洲爪蟾早期原肠胚以高频诱导眼睛形成。体外形成的眼睛在形态上与正常眼睛相似。当将体外形成的眼睛移植到33期蝌蚪中时，可以看到视神经从移植的眼睛延伸到宿主脑的视叶，并且体外眼睛移植在变态后得以保留。此外，我们将体外形成的眼睛移植到双眼被摘除的蝌蚪中。由此产生的幼蛙能够利用移植的眼睛感知亮度，从而控制其皮肤颜色，这表明体外形成的眼睛能够正常发挥功能。
