Khelifi-Touhami Fatima, Taha Ragia A, Badary Osama A, Lezzar Alkassem, Hamada Farid M A
Institute of Natural and Life Sciences, Mentouri University, Constantine, Algeria.
J Biochem Mol Toxicol. 2003;17(6):324-8. doi: 10.1002/jbt.10094.
The effects of three natural phenolic acids (caffeic, ferulic, and p-coumaric) on the rat thyroid gland were examined in a 3-week oral-treatment study. Forty male Wistar albino rats, divided into groups of 10 rats each and fed iodine-rich diet, were administered by gastrointestinal tube saline (control), caffeic acid, ferulic acid, or p-coumaric acid at a dose level of 0.25 micromol/kg/day for 3 weeks. The mean absolute and relative thyroid weights in caffeic, ferulic, or p-coumaric acid groups were significantly increased to 127 and 132%, 146 and 153%, or 189 and 201% compared to control value, respectively. Histological examination of the thyroids of p-coumaric acid group revealed marked hypertrophy and/or hyperplasia of the follicles. Caffeic or ferulic groups showed slight to moderate thyroid gland enlargement. Thyroid lesions in p-coumaric acid group were associated with significant increases in cellular proliferation as indicated by [(3)H]thymidine incorporation. In addition, the goitrogenic effect of p-coumaric acid was further confirmed by significant decreases (50%) in serum tri-iodothyronine (T(3)) and thyroxine (T(4)), and a parallel increase (90%) in serum thyroid stimulating hormone (TSH) compared to control group. These results indicate that administration of p-coumaric acid at relatively high doses induces goiter in rats.
在一项为期3周的口服治疗研究中，检测了三种天然酚酸（咖啡酸、阿魏酸和对香豆酸）对大鼠甲状腺的影响。将40只雄性Wistar白化大鼠分为每组10只的四组，给予富含碘的饮食，并通过胃管分别给予生理盐水（对照组）、咖啡酸、阿魏酸或对香豆酸，剂量为0.25微摩尔/千克/天，持续3周。与对照组相比，咖啡酸、阿魏酸或对香豆酸组的平均绝对甲状腺重量和相对甲状腺重量分别显著增加至127%和132%、146%和153%、189%和201%。对香豆酸组甲状腺的组织学检查显示，滤泡明显肥大和/或增生。咖啡酸或阿魏酸组显示甲状腺轻度至中度肿大。对香豆酸组的甲状腺病变与[³H]胸苷掺入所表明的细胞增殖显著增加有关。此外，与对照组相比，血清三碘甲状腺原氨酸（T₃）和甲状腺素（T₄）显著降低（50%），血清促甲状腺激素（TSH）平行升高（90%），进一步证实了对香豆酸的致甲状腺肿作用。这些结果表明，给予相对高剂量的对香豆酸会诱导大鼠甲状腺肿大。
