Slimane Tounsia Aït, Trugnan Germain
INSERM U538, CHU St-Antoine, 27, rue de Chaligny, 75571 Paris, France.
J Soc Biol. 2003;197(3):211-6.
Polarity is a fundamental characteristic of most eukaryotic cells. The plasma membrane of such cells consists in two structurally and functionally different domains, i.e., the basolateral and the apical membrane, separated by tight junctions. The generation of the distinct molecular identity of both domains and its maintenance in spite of the dynamics of lipids and proteins at either surface requires sophisticated sorting and trafficking mechanisms. Recent progress in the field of polarized trafficking reveals that, for a detailed understanding of its mechanism and regulation, an integrated approach that includes the flow of both lipids and proteins is imperative. In this review, some recent progress in understanding mechanisms involved in protein sorting and trafficking is discussed. We focus on the role of lipid microdomains (Rafts) in trafficking of proteins to the apical surface of polarized cells.
极性是大多数真核细胞的基本特征。此类细胞的质膜由两个结构和功能不同的结构域组成，即基底外侧膜和顶端膜，它们由紧密连接分隔开。尽管两侧表面的脂质和蛋白质具有动态性，但要产生这两个结构域独特的分子特性并维持其稳定性，需要复杂的分选和运输机制。极化运输领域的最新进展表明，为了详细了解其机制和调控，一种包括脂质和蛋白质流动的综合方法势在必行。在这篇综述中，我们讨论了在理解蛋白质分选和运输机制方面的一些最新进展。我们重点关注脂质微区（脂筏）在蛋白质向极化细胞顶端表面运输中的作用。
