Iglesias María Teresa, De Lorenzo Cristina, Del Carmen Polo María, Martín-Alvarez Pedro Jésus, Pueyo Encarnacíon
Instituto Madrileño de Investigación Agraria y Alimentaria, IMIA, Finca El Encín, Apartado 127, 28800 Alcalá de Henares, Spain.
J Agric Food Chem. 2004 Jan 14;52(1):84-9. doi: 10.1021/jf030454q.
With the aim of finding methods that could constitute a solid alternative to melissopalynological and physicochemical analyses to determine the botanical origin (floral or honeydew) of honeys, the free amino acid content of 46 honey samples has been determined. The honeys were collected in a small geographic area of approximately 2000 km(2) in central Spain. Twenty-seven honey samples were classified as floral and 19 as honeydew according to their palynological and physicochemical analyses. The resulting data have been subjected to different multivariant analysis techniques. One hundred percent of honey samples have been correctly classified into either the floral or the honeydew groups, according to their content in glutamic acid and tryptophan. It is concluded that free amino acids are good indicators of the botanical origin of honeys, saving time compared with more tedious analyses.
为了找到能够构成可靠替代方法，以取代通过蜂蜜花粉学和物理化学分析来确定蜂蜜植物来源（花蜜或甘露）的方法，已测定了46个蜂蜜样品的游离氨基酸含量。这些蜂蜜采自西班牙中部一个面积约2000平方公里的小地理区域。根据花粉学和物理化学分析，27个蜂蜜样品被归类为花蜜蜂蜜，19个为甘露蜂蜜。所得数据已采用不同的多变量分析技术进行处理。根据蜂蜜中谷氨酸和色氨酸的含量，100%的蜂蜜样品已被正确归类为花蜜或甘露组。得出的结论是，游离氨基酸是蜂蜜植物来源的良好指标，与更繁琐的分析相比节省了时间。
